Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)
![Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)](https://www.abcam.com/ps/products/154/ab154859/Images/ab154859-339715-ab154859WB2.jpg "Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6177(2)] to ALKBH2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ALKBH2 antibody [EPR6177(2)]
See all ALKBH2 primary antibodies -
Description
Rabbit monoclonal [EPR6177(2)] to ALKBH2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human ALKBH2.
Database link: Q6NS38 -
Positive control
- Human fetal brain, Human testis, 293T, Mouse brain, Mouse heart, Rat brain, Rat heart Jurkat and HeLa lysates;
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General notes
Previously labelled as ABH2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6177(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)](https://www.abcam.com/ps/products/154/ab154859/Images/ab154859-339714-ab154859WB1.jpg)
Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)All lanes : Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution (Purified)
Lane 1 : Human fetal brain lysates
Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Mouse heart lysates
Lane 5 : Rat brain lysates
Lane 6 : Rat heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
-
![Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)](https://www.abcam.com/ps/products/154/ab154859/Images/ab154859-161487-anti-alkbh2-antibody-epr61772-western-blot.jpg)
Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)All lanes : Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution
Lanes 1 & 3 : Human fetal brain tissue lysate
Lane 2 : Human testis tissue lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDa
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![Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)](https://www.abcam.com/ps/products/154/ab154859/Images/ab154859-5-benefits-of-recombinant-antibodies.png)
Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)
