Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6177(2)] to ALKBH2
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ALKBH2 antibody [EPR6177(2)]
    See all ALKBH2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6177(2)] to ALKBH2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human ALKBH2.
    Database link: Q6NS38

  • Positive control

    • Human fetal brain, Human testis, 293T, Mouse brain, Mouse heart, Rat brain, Rat heart Jurkat and HeLa lysates;

  • General notes

     

     

    Previously labelled as ABH2. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)
    Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)
    Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)
    Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)
    All lanes : Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution (Purified)

    Lane 1 : Human fetal brain lysates
    Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 3 : Mouse brain lysates
    Lane 4 : Mouse heart lysates
    Lane 5 : Rat brain lysates
    Lane 6 : Rat heart lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 29 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)
    Western blot - Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)
    All lanes : Anti-ALKBH2 antibody [EPR6177(2)] (ab154859) at 1/1000 dilution

    Lanes 1 & 3 : Human fetal brain tissue lysate
    Lane 2 : Human testis tissue lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 29 kDa

  • Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)
    Anti-ALKBH2 antibody [EPR6177(2)] (ab154859)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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