Anti-HAND1 antibody [OTI1G10] (ab279390)
![Anti-HAND1 antibody [OTI1G10] (ab279390)](https://www.abcam.com/ps/products/279/ab279390/Images/ab279390-2-AntiHAND1-antibody-OTI1G10-westernblot-Testis-Omentum-Uterus-Breast-Brain-LiverOvary-hyroid-gland-colon-human.jpg "Anti-HAND1 antibody [OTI1G10] (ab279390)")
Key features and details
- Mouse monoclonal [OTI1G10] to HAND1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-HAND1 antibody [OTI1G10]
See all HAND1 primary antibodies -
Description
Mouse monoclonal [OTI1G10] to HAND1 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human HAND1 aa 1-215. NP_004812
Database link: O96004 -
Positive control
- Flow Cyt: Jurkat and HeLa cells. WB: Ovary tissue extracts. HEK-293T transfected cells pCMV6-ENTRY HAND1 cDNA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
OTI1G10 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-HAND1 antibody [OTI1G10] (ab279390)All lanes : Anti-HAND1 antibody [OTI1G10] (ab279390) at 1/200 dilution
Lane 1 : Testis tissue extracts
Lane 2 : Omentum tissue extracts
Lane 3 : Uterus tissue extract
Lane 4 : Breast tissue extract
Lane 5 : Brain tissue extract
Lane 6 : Liver tissue extract
Lane 7 : Ovary tissue extract
Lane 8 : Thyroid gland tissue extract
Lane 9 : Colon tissue extract
Lysates/proteins at 10 µg per lane.
Predicted band size: 24 kDa
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![Flow Cytometry - Anti-HAND1 antibody [OTI1G10] (ab279390)](https://www.abcam.com/ps/products/279/ab279390/Images/ab279390-4-AntiHAND1-antibody-OTI1G10-flowcytometry-Jurkat-cells.jpg)
Flow Cytometry - Anti-HAND1 antibody [OTI1G10] (ab279390)Flow cytometric Analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells using ab279390 at a 1/100 dilution (Red), compared to a non-specific negative control antibody (Blue).
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![Flow Cytometry - Anti-HAND1 antibody [OTI1G10] (ab279390)](https://www.abcam.com/ps/products/279/ab279390/Images/ab279390-3-AntiHAND1-antibody-OTI1G10-flowcytometry-HeLa-cells.jpg)
Flow Cytometry - Anti-HAND1 antibody [OTI1G10] (ab279390)Flow cytometric Analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells using ab279390 at a 1/100 dilution (Red), compared to a non-specific negative control antibody (Blue).
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![Western blot - Anti-HAND1 antibody [OTI1G10] (ab279390)](https://www.abcam.com/ps/products/279/ab279390/Images/ab279390-1-AntiHAND1-antibody-OTI1G10-westernblot-HEK293T-cells.jpg)
Western blot - Anti-HAND1 antibody [OTI1G10] (ab279390)All lanes : Anti-HAND1 antibody [OTI1G10] (ab279390) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with the pCMV6-ENTRY control
Lane 2 : HEK-293T transfected cells pCMV6-ENTRY HAND1 cDNA for 48 hrs and lysed
Lysates/proteins at 5 µg per lane.
Predicted band size: 24 kDa