Anti-RNA polymerase II RPB4 antibody (ab229488)
Key features and details
- Rabbit polyclonal to RNA polymerase II RPB4
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNA polymerase II RPB4 antibody
See all RNA polymerase II RPB4 primary antibodies -
Description
Rabbit polyclonal to RNA polymerase II RPB4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey
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Immunogen
Recombinant fragment within Human RNA polymerase II RPB4 (internal sequence). The exact sequence is proprietary.
Database link: O15514 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RNA polymerase II RPB4 antibody (ab229488)All lanes : Anti-RNA polymerase II RPB4 antibody (ab229488) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 16 kDa15% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II RPB4 antibody (ab229488)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RNA polymerase II RPB4 (green) using ab229488 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde/PBS for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
