Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (ab250160)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11750] to POLR2E/rpb5 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free
    See all POLR2E/rpb5 primary antibodies

  • Description

    Rabbit monoclonal [EPR11750] to POLR2E/rpb5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250160 is the carrier-free version of ab180151 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250160 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as POLR2E

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (ab250160)
    Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (ab250160)
    All lanes : Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151) at 1/20000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 25 kDa



    This data was developed using ab180151, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (ab250160)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (ab250160)
    This data was developed using ab180151, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling POLR2E/rpb5 with ab180151 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (ab250160)
    Flow Cytometry - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (ab250160)
    This data was developed using ab180151, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling POLR2E/rpb5 with ab180151 at 1/10 dilution followed by Goat anti rabbit IgG (FITC) at 1/75 dilution (red), compared to a Rabbit IgG control (green).
  • Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (ab250160)
    Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (ab250160)
    Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151) at 1/5000 dilution + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 25 kDa



    This data was developed using ab180151, the same antibody clone in a different buffer formulation.

     

  • Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (ab250160)
    Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free (ab250160)

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