RNA polymerase II CTD Panel (RNA pol II CTD, phospho S2, phospho S5) (ab103968)
Overview
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Product name
RNA polymerase II CTD Panel (RNA pol II CTD, phospho S2, phospho S5) -
Product overview
ab103968 is a RNA polymerase II CTD Panel designed for the validation and characterization of the phosphorylation state of the carboxy-terminal domain (CTD) repeat YSPTSPS of RNA polymerase II. Numerous enzymes, including cell cycle-dependent kinases and TFIIF-dependent phosphatases target the CTD of RNA polymerase II, and the phosphorylation pattern changes during the transcription cycle. S5 phosphorylation occurs in promoter-proximal regions, while S2 phosphorylation predominates in regions that are more distal from the promoter during transcription elongation.
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Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Research areas
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Alternative names
- DNA directed RNA polymerase II A
- DNA-directed RNA polymerase II subunit A
- DNA-directed RNA polymerase II subunit RPB1
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Images
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Western blot - RNA polymerase II CTD Panel (ab103968)Lanes 1 & 3-4 : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) at 1/500 dilution
Lane 2 : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) at 1/2000 dilution
Lanes 1-2 : HeLa nuclear extract
Lane 3 : HeLa nuclear extract withHuman RNA polymerase II CTD repeat YSPTSPS (phospho S5) peptide (ab18488) at 1 µg/ml
Lane 4 : HeLa nuclear extract with S. cerevisiae RNA polymerase II CTD repeat YSPTSPS peptide (ab12795) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution -
ChIP - RNA polymerase II CTD Panel (ab103968)Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab5408 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region. -
ChIP - RNA polymerase II CTD Panel (ab103968)Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 µg of chromatin, 2µg of ab5095 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the inactive AFM and F8 promoters, a region in the GAPDH gene (active) and over the g-Actin gene (active). Schematic diagram of the g-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene. -
ChIP - RNA polymerase II CTD Panel (ab103968)Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 mins. The ChIP was performed with 25 µg of chromatin, 2 µg of ab5131 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the inactive AFM and F8 promoters, the GAPDH promoter (active) and over the y-Actin gene (active). Schematic diagram of the y-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene. -
RNA polymerase II CTD Panel (RNA pol II CTD, phospho S2, phospho S5) (ab103968)
