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Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11750] to POLR2E/rpb5
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-POLR2E/rpb5 antibody [EPR11750]
    See all POLR2E/rpb5 primary antibodies

  • Description

    Rabbit monoclonal [EPR11750] to POLR2E/rpb5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human POLR2E/rpb5 aa 1-100. The exact sequence is proprietary.
    Database link: P19388

  • Positive control

    • HepG2, A431 and HeLa whole cell lysate (ab150035); Human kidney tissue; HeLa cells.

  • General notes

    This product was previously labelled as POLR2E.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
    Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
    All lanes : Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151) at 1/20000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 25 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling POLR2E/rpb5 with ab180151 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
    Flow Cytometry - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling POLR2E/rpb5 with ab180151 at 1/10 dilution followed by Goat anti rabbit IgG (FITC) at 1/75 dilution (red), compared to a Rabbit IgG control (green).

  • Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
    Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
    Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151) at 1/5000 dilution + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 25 kDa

  • Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
    Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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