Human Hemoglobin subunit alpha Antibody Pair - BSA and Azide free (ab241901)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human Hemoglobin subunit alpha Antibody Pair - BSA and Azide free
See all Hemoglobin subunit alpha kits -
Assay type
ELISA set -
Range
500 pg/ml - 32000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human Hemoglobin subunit alpha. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab219049), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Hemoglobin subunit alpha Capture Antibody (unconjugated) 1 x 100µg Human Hemoglobin subunit alpha Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Involved in oxygen transport from the lung to the various peripheral tissues. -
Tissue specificity
Red blood cells. -
Involvement in disease
Heinz body anemias
Alpha-thalassemia
Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
Hemoglobin H disease -
Sequence similarities
Belongs to the globin family. -
Post-translational
modificationsThe initiator Met is not cleaved in variant Thionville and is acetylated. - Information by UniProt
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Alternative names
- Alpha 1 globin
- Alpha globin
- Alpha one globin
see all -
Database links
- Entrez Gene: 3039 Human
- Entrez Gene: 3040 Human
- Omim: 141800 Human
- Omim: 141850 Human
- SwissProt: P69905 Human
- Unigene: 449630 Human
- Unigene: 654744 Human
Images
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Sandwich ELISA - Human Hemoglobin subunit alpha Antibody Pair - BSA and Azide free (ab241901)To learn more about the advantages of recombinant antibodies see here.
