Human Hemoglobin subunit alpha ELISA Kit (ab219049)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 131 pg/ml
- Range: 588 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Hemoglobin subunit alpha ELISA Kit
See all Hemoglobin subunit alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 3.6% Inter-assay Sample n Mean SD CV% Overall 3 14.2% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
131 pg/ml -
Range
588 pg/ml - 20000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 96 84% - 100% Whole Blood 101 81% - 115% Hep Plasma 93 82% - 100% EDTA Plasma 94 91% - 98% Cerebral Spinal Fluid 104 99% - 109% serum free media 88 91% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Hemoglobin subunit alpha ELISA Kit (ab219049) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Hemoglobin subunit alpha protein in cerebral spinal fluid, cit plasma, edta plasma, hep plasma, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Hemoglobin subunit alpha with 131 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Hemoglobin (Haemoglobin) subunits combine to form the quaternary structure of total hemoglobin, the oxygen carrying protein in red blood cells. In normal adults hemoglobin consists of 2 alpha and 2 beta subunits. Hemoglobin alpha subunit can be modified by the glucose on the terminal valine of beta subunits resulting in the Hemoglobin A1C molecule. The resulting A1C molecule is used to measure the average glucose levels in blood over time. ab219049 measures the total Hemoglobin and can recognize both the alpha subunit and A1C modified protein.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Hemoglobin subunit alpha Capture Antibody 1 x 600µl 10X Human Hemoglobin subunit alpha Detector Antibody 1 x 600µl Human Hemoglobin Lyophilized Purified Protein 2 vials Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml 10X Wash Buffer PT (ab206977) 1 x 20ml TMB Development Solution 1 x 12ml Stop Solution 1 x 12ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Plate Seals 1 unit -
Research areas
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Function
Involved in oxygen transport from the lung to the various peripheral tissues. -
Tissue specificity
Red blood cells. -
Involvement in disease
Heinz body anemias
Alpha-thalassemia
Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
Hemoglobin H disease -
Sequence similarities
Belongs to the globin family. -
Post-translational
modificationsThe initiator Met is not cleaved in variant Thionville and is acetylated. - Information by UniProt
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Alternative names
- Alpha 1 globin
- Alpha globin
- Alpha one globin
see all -
Database links
- Entrez Gene: 3039 Human
- Entrez Gene: 3040 Human
- Omim: 141800 Human
- Omim: 141850 Human
- SwissProt: P69905 Human
- Unigene: 449630 Human
- Unigene: 654744 Human
Images
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Other - Human Hemoglobin subunit alpha ELISA Kit (ab219049)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Human Hemoglobin subunit alpha standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Hemoglobin subunit alpha in human serum, plasma, and hemolyzed whole blood samples.The concentrations of Hemoglobin subunit alpha were measured in duplicates, interpolated from the Hemoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (heparin) 25%, plasma (EDTA) 12.5%, and whole blood 1:80,000 . The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hemoglobin subunit alpha concentration was determined to be 0.07 µg/mL in serum, 0.06 µg/mL in plasma (heparin), 0.11 µg/mL in plasma (EDTA), and 400µg/mL in whole blood.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hemoglobin subunit alpha concentration was determined to be 34 ng/mL with a range of 8 – 137 ng/mL.
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Interpolated concentrations of native Hemoglobin subunit alpha in human cerebral spinal fluid sample.The concentrations of Hemoglobin subunit alpha were measured in duplicates, interpolated from the Hemoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: Cerebral spinal fluid 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hemoglobin subunit alpha concentration was determined to be 540 ng/mL in cerebral spinal fluid.
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Interpolated concentrations of spike Hemoglobin subunit alpha in serum free cell culture media sample.The concentrations of Hemoglobin subunit alpha were measured in duplicates, interpolated from the Hemoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: Serum free cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of Natural Native Hemoglobin protein standard and hemoglobin subunit alpha recombinant protein.Each protein was loaded at 5ng/mL and interpolated off the hemoglobin standard curve. The interpolated values are plotted (mean +/- SD, n=2). The observed 4-fold difference is expected, due to the size difference between the alpha subunit and the whole molecule.
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Sandwich ELISA - Human Hemoglobin subunit alpha ELISA Kit (ab219049)To learn more about the advantages of recombinant antibodies see here.
