Anti-ERM / Etv5 antibody (ab102010)
Key features and details
- Rabbit polyclonal to ERM / Etv5
- Suitable for: ICC, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ERM / Etv5 antibody
See all ERM / Etv5 primary antibodies -
Description
Rabbit polyclonal to ERM / Etv5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P MouseHumanWB Human
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Immunogen
Recombinant fragment corresponding to Human ERM/ Etv5 aa 244-404.
Database link: P41161
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ERM / Etv5 antibody (ab102010)All lanes : Anti-ERM / Etv5 antibody (ab102010) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T whole cell extracts
Lane 2 : Transfected HEK-293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 58 kDa10% SDS-PAGE
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Immunocytochemistry - Anti-ERM / Etv5 antibody (ab102010)ab102010 staining ERM / Etv5 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody (1/500) and cytoskeleton stained red with Phalloidin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERM / Etv5 antibody (ab102010)ab102010 staining ERM / Etv5 in Mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/500).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERM / Etv5 antibody (ab102010)Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ab102010 at 1/100 dilution. -
Western blot - Anti-ERM / Etv5 antibody (ab102010)Anti-ERM / Etv5 antibody (ab102010) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 58 kDa
7.5% SDS PAGE
