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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Signaling Pathway Nuclear Signaling STATs

Anti-STAT6 antibody (ab227497)

Anti-STAT6 antibody (ab227497)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to STAT6
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-STAT6 antibody
    See all STAT6 primary antibodies
  • Description

    Rabbit polyclonal to STAT6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human STAT6 (internal sequence). The exact sequence is proprietary.
    Database link: P42226

  • Positive control

    • IP: HeLa whole cell lysate. IHC-P: Mouse kidney tissue; C2C12 xenograft tissue. WB: HepG2 whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • STAT family
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • In Cell ELISA Kits
    • Phosphoprotein ICE kits

Images

  • Western blot - Anti-STAT6 antibody (ab227497)
    Western blot - Anti-STAT6 antibody (ab227497)
    Anti-STAT6 antibody (ab227497) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 94 kDa



    7.5% SDS-PAGE gel.

  • Immunoprecipitation - Anti-STAT6 antibody (ab227497)
    Immunoprecipitation - Anti-STAT6 antibody (ab227497)

    STAT6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2 µg ab227497. Western blot was performed from the immunoprecipitate using ab227497. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: HeLa whole cell lysate 50 μg.

    Lane 2: Control IP in HeLa whole cell lysate with 2 μg of preimmune rabbit IgG.

    Lane 3: ab227497 IP in HeLa whole cell lysate.

    10% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody (ab227497)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody (ab227497)

    Paraffin-embedded mouse kidney tissue stained for STAT6 using ab227497 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody (ab227497)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody (ab227497)

    Paraffin-embedded C2C12 (mouse) xenograft tissue stained for STAT6 using ab227497 at 1/500 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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