Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TCF-4 antibody [NCI-R159-6] - BSA and Azide free (ab223073)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [NCI-R159-6] to TCF-4 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IHC, ChIP
  • Reacts with: Human

Overview

  • Product name

    Anti-TCF-4 antibody [NCI-R159-6] - BSA and Azide free
    See all TCF-4 primary antibodies

  • Description

    Rabbit monoclonal [NCI-R159-6] to TCF-4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC, ChIPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab223073 is the carrier-free version of ab217668 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab223073 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    For detailed protocol using this antibody for IHC, ChIP, and Flow Cyt, please refer to the following paper:
    A Druggable TCF4- and BRD4-Dependent Transcriptional Network Sustains Malignancy in Blastic Plasmacytoid Dendritic Cell Neoplasm

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • ChIP - Anti-TCF-4 antibody [NCI-R159-6] - BSA and Azide free (ab223073)
    ChIP - Anti-TCF-4 antibody [NCI-R159-6] - BSA and Azide free (ab223073)

    Cal-1 cells (Human plasmacytoid dendritic cell line) were cross-linked with 1% formaldehyde for 5 min at RT. Cross-linked cells were first washed with ice-cold PBS and then resuspended in ice-cold RIPA buffer (10mM Tris-HCl pH8, 140 mM NaCl, 1mM EDTA pH 8, 0.5 mM EGTA, 1% Triton X-100, 0.1% SDS and 0.1% Sodium Deoxycholate) to a final concentration of 5x106 cells/ml. DNA was sheared with a Misonix XL sonicator, by performing 12 x 45’’ sonication cycles at power setting of 5. For each ChIP reaction, 2x107 chromatin cell equivalents were incubated overnight with10 μg of ab217668. The following day, chromatin/antibody complexes were incubated with 50 μl of Protein G/Protein A magnetic beads mix (G to A ratio 3:1) for 4 h at 4°C. Normal rabbit IgG was added to the beads as control.

    The TCF-4 locus ChIP-seq tracks for BRD4 (blue), RNA Pol2 (red), and TCF-4 (green) are shown for Cal-1 cells. This data was kindly provided by our collaborator Dr. Louis M. Staudt, and has been published (PMID: 27846392).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217668).

  • Flow Cytometry - Anti-TCF-4 antibody [NCI-R159-6] - BSA and Azide free (ab223073)
    Flow Cytometry - Anti-TCF-4 antibody [NCI-R159-6] - BSA and Azide free (ab223073)

    Flow cytometric analysis of 1% paraformaldehyde-fixed, ice-cold methanol permeabilized Cal-1 cells (Human plasmacytoid dendritic cell line) (black - positive control) and Cal-1 cells infected with either Ctrl (left green) or TCF4 (right green) shRNA, labeling TCF4 with ab217668 at 1/100 dilution (green and black) compared with a Rabbit IgG control (grey). Goat anti-Rabbit IgG (Alexa Fluor® 647) at 1/500 dilution was used as the secondary antibody.

    The data was provided by our collaborator Dr. Louis M. Staudt, and published in Cancer Cell 30, 764-778, 2016 (PMID: 27846392). Several TCF4 shRNAs were used. This FC image shows data for shRNA TCF4 #1.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217668).

  • Immunohistochemistry - Anti-TCF-4 antibody [NCI-R159-6] - BSA and Azide free (ab223073)
    Immunohistochemistry - Anti-TCF-4 antibody [NCI-R159-6] - BSA and Azide free (ab223073)

    Immunohistochemical analysis of 4% Formalin fixed Blastic plasmacytoid dendritic cell neoplasm (BPDCN) cell pellets after selection and induction of shRNA expression for 1 day, labeling TCF-4 with ab217668 at 1/100 dilution. Universal DAB Detection Kit was used for detection of IHC staining on an automated system.

    The data was provided by our collaborator Dr. Louis M. Staudt, and published in Cancer Cell 30, 764-778, 2016 (PMID: 27846392). Several TCF-4 shRNAs were used. This IHC image shows data for shRNA TCF-4 #2.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217668).

  • Immunohistochemistry - Anti-TCF-4 antibody [NCI-R159-6] - BSA and Azide free (ab223073)
    Immunohistochemistry - Anti-TCF-4 antibody [NCI-R159-6] - BSA and Azide free (ab223073)

    Immunohistochemical analysis of 4% Formalin fixed human tonsil labeling TCF-4 with ab217668 at 1/100 dilution. Universal DAB Detection Kit was used for detection of IHC staining on an automated system.

    pDCs: plasmacytoid dendritic cells.

    GC: Germinal Center.

    The data was provided by our collaborator Dr. Louis M. Staudt, and published in Cancer Cell 30, 764-778, 2016 (PMID: 27846392).

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217668).

  • Anti-TCF-4 antibody [NCI-R159-6] - BSA and Azide free (ab223073)
    Anti-TCF-4 antibody [NCI-R159-6] - BSA and Azide free (ab223073)

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