Anti-SOX8 antibody (ab104245)
Key features and details
- Rabbit polyclonal to SOX8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SOX8 antibody
See all SOX8 primary antibodies -
Description
Rabbit polyclonal to SOX8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide corresponding to Human SOX8 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab125935) -
Positive control
- This antibody gave a positive signal in both Human Testis and Human Colon tissue lysates.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatCowDogMacaque monkeyGorillaApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).Target
-
Function
May play a role in central nervous system, limb and facial development. May be involved in male sex determination. Binds the consensus motif 5'-[AT][AT]CAA[AT]G-3'. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 30812 Human
- Entrez Gene: 20681 Mouse
- Entrez Gene: 302993 Rat
- Omim: 605923 Human
- SwissProt: P57073 Human
- SwissProt: Q04886 Mouse
- Unigene: 243678 Human
- Unigene: 258220 Mouse
-
Alternative names
- MGC24837 antibody
- SOX 8 antibody
- Sox8 antibody
see all
Images
-
Western blot - Anti-SOX8 antibody (ab104245)All lanes : Anti-SOX8 antibody (ab104245) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
Datasheets and documents
References (2)
ab104245 has been referenced in 2 publications.
- Kimura S et al. Sox8 is essential for M cell maturation to accelerate IgA response at the early stage after weaning in mice. J Exp Med 216:831-846 (2019). PubMed: 30877171
- Gryder BE et al. Histone hyperacetylation disrupts core gene regulatory architecture in rhabdomyosarcoma. Nat Genet 51:1714-1722 (2019). PubMed: 31784732
Images
-
Western blot - Anti-SOX8 antibody (ab104245)All lanes : Anti-SOX8 antibody (ab104245) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
