Anti-TCF12 antibody [OTI4B5] - BSA and Azide free (ab273665)
![Anti-TCF12 antibody [OTI4B5] - BSA and Azide free (ab273665)](https://www.abcam.com/ps/products/273/ab273665/Images/ab273665-394512-AntiTCF12antibodyOTI4B5westernblotJurkatK562A549MCF7HeLahuman.jpg "Anti-TCF12 antibody [OTI4B5] - BSA and Azide free (ab273665)")
Key features and details
- Mouse monoclonal [OTI4B5] to TCF12 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-TCF12 antibody [OTI4B5] - BSA and Azide free
See all TCF12 primary antibodies -
Description
Mouse monoclonal [OTI4B5] to TCF12 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length protein corresponding to Human TCF12. (NP_996919) produced in E.coli.
Database link: Q99081 -
Positive control
- WB: Jurkat, K562, A549, MCF7 and HeLa cell extracts. HEK-293T transfected with pCMV6-ENTRY TCF12 cDNA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
OTI4B5 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-TCF12 antibody [OTI4B5] - BSA and Azide free (ab273665)All lanes : Same antibody clone in a different buffer formulation at 1/500 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell extract
Lane 3 : A549 (Human lung carcinoma cell line) cell extract
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) cell extract
Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 72 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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![Western blot - Anti-TCF12 antibody [OTI4B5] - BSA and Azide free (ab273665)](https://www.abcam.com/ps/products/273/ab273665/Images/ab273665-394509-AntiTCF12antibodyOTI4B5westernblottransfectedHEK293T.jpg)
Western blot - Anti-TCF12 antibody [OTI4B5] - BSA and Azide free (ab273665)All lanes : Same antibody clone in a different buffer formulation at 1/2000 dilution
Lane 1 : HEK-293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY TCF12 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 72 kDaCells were transfected for 48 hours then lysed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.