Anti-Alpha Dystroglycan antibody [2238] (ab106110)
![Anti-Alpha Dystroglycan antibody [2238] (ab106110)](https://www.abcam.com/ps/products/106/ab106110/Images/ab106110-457225-anti-alpha-dystroglycan-antibody-2238-western-blot-human-brain-shsy5y-mouse-brain-mouse-heart.jpg "Anti-Alpha Dystroglycan antibody [2238] (ab106110)")
Key features and details
- Mouse monoclonal [2238] to Alpha Dystroglycan
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Cow, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Alpha Dystroglycan antibody [2238]
See all Alpha Dystroglycan primary antibodies -
Description
Mouse monoclonal [2238] to Alpha Dystroglycan -
Host species
Mouse -
Specificity
ab106110 is specific for a glycoepitope present on brain Alpha Dystroglycan. The clone was generated using enriched bovine brain Alpha dystroglycan, and has been shown to bind Alpha Dystroglycan from enriched mouse, rat, rabbit and bovine brain tissues but did not bind Alpha Dystroglycan from striated muscle, peripheral nerve or kidney (PMID: 16709410).
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Cow, Human -
Immunogen
Other Immunogen Type corresponding to Cow Alpha Dystroglycan. Glycoepitope on brain bovine alpha-dystroglycan.
Database link: O18738 -
Positive control
- WB: Human brain tissue lysate; SH-SY5Y cell lysate; Mouse brain tissue lysate; Rat brain tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
0.2 µm filtered antibody solution -
Clonality
Monoclonal -
Clone number
2238 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-Alpha Dystroglycan antibody [2238] (ab106110)All lanes : Anti-Alpha Dystroglycan antibody [2238] (ab106110) at 1/100 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Mouse heart tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 75-100 kDa why is the actual band size different from the predicted?Lanes 1 - 6: Merged signal (red and green). Green - ab106110 observed at 75-100 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.
ab106110 was shown to react with DAG1 in Western blot. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab106110 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at a 1 in 100 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
