Anti-Sohlh1 antibody (ab41520)
Key features and details
- Rabbit polyclonal to Sohlh1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Sohlh1 antibody -
Description
Rabbit polyclonal to Sohlh1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Sohlh1.
Read Abcam's proprietary immunogen policy (Peptide available as ab42012.) -
Positive control
- This antibody gave a positive signal in the following Mouse Tissue lysates: Fetus (14 Day Old), Ovary (data not shown)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseApplication Abreviews Notes WB Use a concentration of 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).Notes WB
Use a concentration of 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).Target
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Function
Probable transcription factor required during spermatogenesis and oogenesis. -
Involvement in disease
Note=Genetic variations in SOHLH1 may be associated with non-obstructive azoospermia. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 227631 Mouse
- SwissProt: Q6IUP1 Mouse
- Unigene: 301632 Mouse
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Alternative names
- GM110 antibody
- Helix Loop Helix Protein TOHLH1 antibody
- Sohlh1 antibody
see all
Images
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Western blot - Anti-Sohlh1 antibody (ab41520)Anti-Sohlh1 antibody (ab41520) at 2 µg/ml + Mouse Fetus (14 Day Old) Tissue Lysate at 20 µg
Secondary
Goat polyclonal to rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 130 kDa, 73 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
References (3)
ab41520 has been referenced in 3 publications.
- Liu G et al. Primordial follicle activation is affected by the absence of Sohlh1 in mice. Mol Reprod Dev 86:20-31 (2019). PubMed: 30358927
- Esakky P et al. Testicular cells exhibit similar molecular responses to cigarette smoke condensate ex vivo and in vivo. FASEB J 32:63-72 (2018). PubMed: 28842431
- Desimio MG et al. SOHLH1 and SOHLH2 directly down-regulate STIMULATED BY RETINOIC ACID 8 (STRA8) expression. Cell Cycle 14:1036-45 (2015). PubMed: 25603532
Images
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Western blot - Anti-Sohlh1 antibody (ab41520)Anti-Sohlh1 antibody (ab41520) at 2 µg/ml + Mouse Fetus (14 Day Old) Tissue Lysate at 20 µg
Secondary
Goat polyclonal to rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 130 kDa, 73 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
