Anti-Integrin alpha 4/CD49D antibody [HP2/1] (ab22858)
![Anti-Integrin alpha 4/CD49D antibody [HP2/1] (ab22858)](https://www.abcam.com/ps/products/22/ab22858/Images/ab22858-287538-anti-integrin-alpha-4-antibody-hp2-1-immunofluorescence.jpg "Anti-Integrin alpha 4/CD49D antibody [HP2/1] (ab22858)")
Key features and details
- Mouse monoclonal [HP2/1] to Integrin alpha 4/CD49D
- Suitable for: ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Integrin alpha 4/CD49D antibody [HP2/1]
See all Integrin alpha 4/CD49D primary antibodies -
Description
Mouse monoclonal [HP2/1] to Integrin alpha 4/CD49D -
Host species
Mouse -
Specificity
This antibody recognises the 80kD alpha chain of VLA-4. Reacts with monocytes, T cells, B cells, thymocytes and Langerhans cells. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Tissue, cells or virus corresponding to Human Integrin alpha 4/CD49D.
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Positive control
- ICC/IF: wildtype HAP-1 cells.
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General notes
Can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure. HP2/1 inhibits cell binding to soluble VCAM-1.
This product was previously labelled as Integrin alpha 4
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
Can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure. HP2/1 inhibits cell binding to soluble VCAM-1. -
Clonality
Monoclonal -
Clone number
HP2/1 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 4/CD49D antibody [HP2/1] (ab22858)
ab22858 staining Integrin alpha 4/CD49D in wild-type HAP1 cells (top panel) and Integrin alpha 4/CD49D knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab22858 at 5μg/ml concentration and ab202272 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
