Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab70547)
Key features and details
- Rabbit polyclonal to Cellular Apoptosis Susceptibility/CSE1L
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cellular Apoptosis Susceptibility/CSE1L antibody
See all Cellular Apoptosis Susceptibility/CSE1L primary antibodies -
Description
Rabbit polyclonal to Cellular Apoptosis Susceptibility/CSE1L -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide corresponding to Human Cellular Apoptosis Susceptibility/CSE1L (N terminal).
Database link: NP_001307.2 -
Positive control
- WB: HeLa and Ramos whole cell lysate. IP: HEK-293T whole cell lysate. IHC-P: Human testicular seminoma; mouse plasmacytoma.
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General notes
This product was previously labelled as Cellular Apoptosis Susceptibility
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab70547) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from Ramos cells at 50 µg
Developed using the ECL technique.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 3 minutes
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Paraffin embedded mouse plasmacytoma tissue stained for CSE1L using ab70547 at 0.2 µg/ml in immunohistochemical analysis.
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Paraffin embedded human testicular seminoma tissue stained for CSE1L using ab70547 at 0.2 µg/ml in immunohistochemical analysis.
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Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK-293T cells prepared using NETN lysis buffer.
Affinity purified rabbit anti-CSE1 antibody used for IP at 6 µg per reaction. CSE1 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-CSE1 antibody BL1977.
For blotting immunoprecipitated CSE1, ab70547 was used at 1 µg/ml. Chemiluminescence had an exposure time of 10 seconds.