Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell lysate (ab257590)
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab261016 - Human PLCG1 knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
-
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
-
Western blot - Human PLCG1 knockout HEK293T cell lysate (ab257590)
Lane 1: Wild-type HEK-293T cell lysate (40ug)
Lane 2: PLCG1 knockout HEK-293T cell lysate (40ug)
Lanes 1- 2: Merged signal (red and green). Green - ab109501 observed at 160 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109501 Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] was shown to specifically react with Phospholipase C gamma 1/PLC-gamma-1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266530 (knockout cell lysate ab257590) was used. Wild-type and Phospholipase C gamma 1/PLC-gamma-1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109501 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Sanger Sequencing - Human PLCG1 knockout HEK293T cell lysate (ab257590)Homozygous: 267 bp deletion in exon1
