Anti-MAML1 antibody (ab65090)
Key features and details
- Rabbit polyclonal to MAML1
- Suitable for: IP, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MAML1 antibody
See all MAML1 primary antibodies -
Description
Rabbit polyclonal to MAML1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Horse
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus MAML1.
Read Abcam's proprietary immunogen policy (Peptide available as ab66491.) -
Positive control
- This antibody gave a positive signal in the following Lysates; SW480, HepG2, HeLa and Rat Thymus Tissue Lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab65090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IP Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 117 kDa (predicted molecular weight: 108 kDa). ICC/IF Use a concentration of 1 µg/ml. Target
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Function
Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. -
Tissue specificity
Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen. -
Sequence similarities
Belongs to the mastermind family. -
Domain
The C-terminal region is required for transcriptional activation. -
Cellular localization
Nucleus speckle. Nuclear, in a punctate manner. - Information by UniProt
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Database links
- Entrez Gene: 9794 Human
- Entrez Gene: 103806 Mouse
- Entrez Gene: 303101 Rat
- Omim: 605424 Human
- SwissProt: Q92585 Human
- SwissProt: Q6T264 Mouse
- Unigene: 631951 Human
- Unigene: 51116 Mouse
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Alternative names
- Mam-1 antibody
- Mam1 antibody
- MAML 1 antibody
see all
Images
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Western blot - Anti-MAML1 antibody (ab65090)All lanes : Anti-MAML1 antibody (ab65090) at 1 µg/ml
Lane 1 :SW480 whole cell lysate (ab3957)
Lane 2 : Rat Thymus Tissue Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 108 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-MAML1 antibody (ab65090)MAML1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MAML1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65090.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 117kDa; MAML1
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Immunocytochemistry/ Immunofluorescence - Anti-MAML1 antibody (ab65090)ICC/IF image of ab65090 stained HeLa cells. The cells were fixed with 4% PFA (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65090 at 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 1µg/ml. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 1µg/ml.
Protocols
Datasheets and documents
References (1)
ab65090 has been referenced in 1 publication.
- Hsu YC et al. De-Glycyrrhizinated Licorice Extract Attenuates High Glucose-Stimulated Renal Tubular Epithelial-Mesenchymal Transition via Suppressing the Notch2 Signaling Pathway. Cells 9:N/A (2020). PubMed: 31948095
Images
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Western blot - Anti-MAML1 antibody (ab65090)All lanes : Anti-MAML1 antibody (ab65090) at 1 µg/ml
Lane 1 :SW480 whole cell lysate (ab3957)
Lane 2 : Rat Thymus Tissue Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 108 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-MAML1 antibody (ab65090)
MAML1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MAML1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65090.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 117kDa; MAML1
-
Immunocytochemistry/ Immunofluorescence - Anti-MAML1 antibody (ab65090)ICC/IF image of ab65090 stained HeLa cells. The cells were fixed with 4% PFA (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65090 at 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 1µg/ml. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 1µg/ml.
