Anti-CXCL11 antibody [EPR23814-16] (ab259863)
![Anti-CXCL11 antibody [EPR23814-16] (ab259863)](https://www.abcam.com/ps/products/259/ab259863/Images/ab259863-459602-anti-cxcl11-antibody-epr2381416-western-blot-murine.jpg "Anti-CXCL11 antibody [EPR23814-16] (ab259863)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23814-16] to CXCL11
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-CXCL11 antibody [EPR23814-16]
See all CXCL11 primary antibodies -
Description
Rabbit monoclonal [EPR23814-16] to CXCL11 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: RAW 264.7 (treated with 200 ng/ml interferon-gamma for 4 h, then 300 ng/ml Brefeldin A (BFA) for 20 h) and RAW 264.7 (treated with 80 nM PMA for 24 h, then with 100 ng/ml LPS for 4 h, then 300 ng/ml Brefeldin A (BFA) for 3 h) whole cell lysates; Mouse pancreas tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23814-16 -
Isotype
IgG
Images
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Western blot - Anti-CXCL11 antibody [EPR23814-16] (ab259863)All lanes : Anti-CXCL11 antibody [EPR23814-16] (ab259863) at 1/2000 dilution
Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 2 : RAW264.7 treated with 200 ng/ml interferon-gamma for 4 hours, then 300 ng/ml Brefeldin A (BFA) was added to the treated cells for 20 hours, whole cell lysate
Lane 3 : RAW264.7 whole cell lysate
Lane 4 : RAW264.7 treated with 80 nM PMA for 24 hours, then treated with 100 ng/ml LPS for 4 hours, then 300 ng/ml Brefeldin A (BFA) was added to the treated cells for 3 hours, whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 10 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 26 seconds.
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![Western blot - Anti-CXCL11 antibody [EPR23814-16] (ab259863)](https://www.abcam.com/ps/products/259/ab259863/Images/ab259863-459603-anti-cxcl11-antibody-epr2381416-western-blot-lysate.jpg)
Western blot - Anti-CXCL11 antibody [EPR23814-16] (ab259863)All lanes : Anti-CXCL11 antibody [EPR23814-16] (ab259863) at 1/2000 dilution
Lane 1 : Mouse pancreas tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 10 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: skeletal muscle.
Exposure time: 26 seconds.
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(ab259863)
