Anti-Gli2 antibody [9HCLC] (ab277800)
![Anti-Gli2 antibody [9HCLC] (ab277800)](https://www.abcam.com/ps/products/277/ab277800/Images/ab277800-1-antiGLI2recombinantpolyclonalantibody9hclcWB.jpg "Anti-Gli2 antibody [9HCLC] (ab277800)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [9HCLC] to Gli2
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Gli2 antibody [9HCLC]
See all Gli2 primary antibodies -
Description
Rabbit recombinant multiclonal [9HCLC] to Gli2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Gli2 aa 1-350.
Database link: P10070 -
Positive control
- WB: MDA-MB-231, MDA-MB-231 treated with TGF-ß1 (Serum starved and treated with 5 ng/mL for 48hr) , MCF-7, MCF-7 treated with TGF-ß1 (Serum starved and treated with 5 ng/mL for 48hr), A549, A549 treated with TGF-ß1 (Serum starved and treated with 5 ng/mL for 48hr). Mouse pancreas, Rat Pancreas and Mouse Kidney tissue extracts.
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
9HCLC -
Isotype
IgG
Images
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Western blot - Anti-Gli2 antibody (ab277800)All lanes : Anti-Gli2 antibody [9HCLC] (ab277800) at 2 µg/ml
Lane 1 : MDA-MB-231 nuclear enriched extracts
Lane 2 : MDA-MB-231 treated with TGF-ß1 (Serum starved and treated with 5 ng/mL for 48hr) nuclear enriched extracts
Lane 3 : MCF-7 nuclear enriched extracts
Lane 4 : MCF-7 treated with TGF-ß1 (Serum starved and treated with 5 ng/mL for 48hr) nuclear enriched extracts
Lane 5 : A549 nuclear enriched extracts
Lane 6 : A549 treated with TGF-ß1 (Serum starved and treated with 5 ng/mL for 48hr) nuclear enriched extracts
Lane 7 : Mouse pancreas tissue extract
Lane 8 : Rat Pancreas tissue extract
Lane 9 : Mouse Kidney tissue extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal HRP conjugate at 1/2500 dilution
Predicted band size: 167 kDa
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![Anti-Gli2 antibody [9HCLC] (ab277800)](https://www.abcam.com/ps/products/277/ab277800/Images/ab277800-2-benefits-of-recombinant-antibodies.jpg)
Anti-Gli2 antibody [9HCLC] (ab277800)