Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade (ab191601)
![Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade (ab191601)](https://www.abcam.com/ps/products/191/ab191601/Images/ab191601-415162-anti-stat2-phospho-y690-antibody-epr1854942-chip-a431-cells.JPG "Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade (ab191601)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18549-42] to STAT2 (phospho Y690) - ChIP Grade
- Suitable for: WB, IP, ChIP, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade
See all STAT2 primary antibodies -
Description
Rabbit monoclonal [EPR18549-42] to STAT2 (phospho Y690) - ChIP Grade -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanIP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 (starved overnight, then 10 ng/ml IFN-alpha treated for 30 minutes) whole cell lysate. IP: K562 (treated with 2500 U/ml IFN alpha for 30 mins) whole cell lysate. ChIP: Chromatin prepared from A431 cells (starved overnight, treated with IFN-alpha (10ng/ml 0.5 h).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18549-42 -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] (ab191601)
Chromatin was prepared from A431 (human epidermoid carcinoma cell line)(starved overnight, treated with IFN-α (10ng/ml 0.5 h)) and A431 (starved overnight) (non-treated) according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 µg of chromatin, 5 µg of ab191601 (Red), or 5 µg of rabbit normal IgG ab172730 (Gray) and 25 µl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).Primers are from paper PMID:30240626.
*https://www.abcam.com/resources?keywords=X%20ChIP%20protocol
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Western blot - Anti-STAT2 (phospho Y690) antibody (ab191601)All lanes : Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade (ab191601) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma epithelial cell) (starved overnight, then 10 ng/ml IFN-alpha treated for 30 minutes), whole cell lysate
Lane 2 : A431 (starved overnight), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 97 kDa
Observed band size: 113,97 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 123 seconds.
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Immunoprecipitation - Anti-STAT2 (phospho Y690) antibody (ab191601)STAT2 (phospho Y690) was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia) (treated with 2500 U/ml IFN alpha for 30 mins) whole cell lysate with ab191601 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab191601 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/1000 dilution.
Lane 1: K-562 (human chronic myelogenous leukemia) (treated as above) whole cell lysate 10 ug
Lane 2: ab191601 IP in K-562 (treated as above) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191601 in K-562 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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Dot Blot - Anti-STAT2 (phospho Y690) antibody (ab191601)Dot blot analysis of STAT2 (phospho Y690) using ab191601 at 1/1000 dilution (1.4 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.
Lane 1: STAT2 (phospho Y690) phospho peptide
Lane 2: STAT2 non-phospho peptideExposure time: 30 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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![Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade (ab191601)](https://www.abcam.com/ps/products/191/ab191601/Images/ab191601-447349-benefitsofrecombinantantibodies.png)
Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade (ab191601)