Anti-STAT1 antibody [42/Stat1] (ab280210)
![Anti-STAT1 antibody [42/Stat1] (ab280210)](https://www.abcam.com/ps/products/280/ab280210/Images/ab280210-455121-anti-stat1-antibody-42stat1-western-blot-a431.jpg "Anti-STAT1 antibody [42/Stat1] (ab280210)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [42/Stat1] to STAT1
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-STAT1 antibody [42/Stat1]
See all STAT1 primary antibodies -
Description
Mouse monoclonal [42/Stat1] to STAT1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431. A549. MCF7 and HEK-293 whole cell lysate. HeLa treated with 10ng/ml IFN-gamma for 24 hours. THP-1 treated with 50ng/ml IFN alpha for 24 hours. IP: A431 whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
42/Stat1 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-STAT1 antibody [42/Stat1] (ab280210)All lanes : Anti-STAT1 antibody [42/Stat1] (ab280210) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
Lane 2 : A549 (Human lung carcinoma epithelial cell ), whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 87 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-STAT1 antibody [42/Stat1] (ab280210)](https://www.abcam.com/ps/products/280/ab280210/Images/ab280210-455122-anti-stat1-antibody-42stat1-western-blot-untreated-hela.jpg)
Western blot - Anti-STAT1 antibody [42/Stat1] (ab280210)All lanes : Anti-STAT1 antibody [42/Stat1] (ab280210) at 1/1000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
Lane 2 : HeLa treated with 10ng/ml IFN-gamma for 24 hours, whole cell lysate
Lane 3 : Untreated THP-1 (human monocytic leukemia monocyte), whole cell lysate
Lane 4 : THP-1 treated with 50ng/ml IFN alpha for 24 hours, added 10 µM MG-132 for 6 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 87 kDa
Exposure time: 8 secondsThe expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 19478064, 26859759).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-STAT1 antibody [42/Stat1] (ab280210)](https://www.abcam.com/ps/products/280/ab280210/Images/ab280210-1-anti-stat1-antibody-42stat1-immunoprecipitation-a431.jpg)
Immunoprecipitation - Anti-STAT1 antibody [42/Stat1] (ab280210)STAT1 was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma) whole cell lysate 10 µg with ab280210 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280210 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.
Lane 1: A431 whole cell lysate 10 µg
Lane 2: ab280210 IP in A431 whole cell lysate
Lane 3: Mouse monoclonal IgG2b instead of ab280210 in A431 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds.
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![Anti-STAT1 antibody [42/Stat1] (ab280210)](https://www.abcam.com/ps/products/280/ab280210/Images/ab280210-455158-benefitsofrecombinantantibodies.jpg)
Anti-STAT1 antibody [42/Stat1] (ab280210)
