Anti-MDMX/MDM4 (phospho S367) antibody [15] (ab122926)
![Anti-MDMX/MDM4 (phospho S367) antibody [15] (ab122926)](https://www.abcam.com/ps/products/122/ab122926/Images/ab122926-136256-anti-mdmx-phospho-s367-antibody-15-western-blot.jpg "Anti-MDMX/MDM4 (phospho S367) antibody [15] (ab122926)")
Key features and details
- Mouse monoclonal [15] to MDMX/MDM4 (phospho S367)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-MDMX/MDM4 (phospho S367) antibody [15]
See all MDMX/MDM4 primary antibodies -
Description
Mouse monoclonal [15] to MDMX/MDM4 (phospho S367) -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MDMX/MDM4.
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Positive control
- MCF cells reincubated with the proteasome inhibitor MG132 (20 uM) and exposed to DNA damaging agent, adriamycin (3 uM) or etoposide (20 uM).
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General notes
This product was previously labelled as MDMX
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 6
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
15 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-MDMX/MDM4 (phospho S367) antibody [15] (ab122926)All lanes : Anti-MDMX/MDM4 (phospho S367) antibody [15] (ab122926) at 1 µg/ml
Lane 1 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM)
Lane 2 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 1 hour
Lane 3 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 2 hour
Lane 4 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 3 hour
Lane 5 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 4 hour
Lane 6 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 1 hour
Lane 7 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 2 hour
Lane 8 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 3 hour
Lane 9 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 4 hour
Predicted band size: 55 kDaThe upper lanes show incubation with a non-phosphos antibody to MDMX/MDM4. The lower lanes as indicated above.
The cell lysates were immunoprecipitated with another anti-MDMX/MDM4 antibody before western blotting.
