Anti-CD11a antibody (ab78058)
Key features and details
- Rabbit polyclonal to CD11a
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD11a antibody
See all CD11a primary antibodies -
Description
Rabbit polyclonal to CD11a -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human CD11a aa 950-1050 conjugated to keyhole limpet haemocyanin.
(Peptide available asab90246) -
Positive control
- This antibody gave a positive signal in the following Human tissue lysates: Thymus; Spleen.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P HumanWB HumanAll applications ChimpanzeeApplication Abreviews Notes IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.WB Use a concentration of 1 µg/ml. Detects a band of approximately 161 kDa (predicted molecular weight: 129 kDa).Notes IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 161 kDa (predicted molecular weight: 129 kDa).Target
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Function
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. -
Tissue specificity
Leukocytes. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3683 Human
- Omim: 153370 Human
- SwissProt: P20701 Human
- Unigene: 174103 Human
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Alternative names
- Lymphocyte function associated antigen, type 1, alpha subunit antibody
- Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
- Antigen CD11A antibody
see all
Images
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Western blot - Anti-CD11a antibody (ab78058)All lanes : Anti-CD11a antibody (ab78058) at 1 µg/ml
Lane 1 : Human thymus tissue lysate - total protein (ab30146)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 129 kDa
Observed band size: 161 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 46 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
CD11A contains an exstensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight (161 kDa) than predicted (129 kDa). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody (ab78058)IHC image of CD11a staining in Human Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78058, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Protocols
Datasheets and documents
References (0)
ab78058 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-CD11a antibody (ab78058)All lanes : Anti-CD11a antibody (ab78058) at 1 µg/ml
Lane 1 : Human thymus tissue lysate - total protein (ab30146)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 129 kDa
Observed band size: 161 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 46 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
CD11A contains an exstensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight (161 kDa) than predicted (129 kDa). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody (ab78058)IHC image of CD11a staining in Human Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78058, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
