Antibodies, Proteins, Kits and Reagents for Life Science

300 865 ₸ Product size

100 µl 300 865 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CD11b antibody [EPR19387] (ab184308)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19387] to CD11b
Suitable for: WB, ICC/IF, IP, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-CD11b antibody [EPR19387]
See all CD11b primary antibodies
Description

Rabbit monoclonal [EPR19387] to CD11b
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: J774A.1, M1 and RAW 264.7 whole cell lysates. ICC/IF: RAW 264.7 cells. Flow Cyt: RAW 264.7 cells. IP: RAW 264.7 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19387
Isotype

IgG
Research areas

Flow Cytometry - Anti-CD11b antibody [EPR19387] (ab184308)

Flow cytometric analysis of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling CD11b with ab184308 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] -Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-CD11b antibody [EPR19387] (ab184308)

All lanes : Anti-CD11b antibody [EPR19387] (ab184308) at 1/1000 dilution

Lane 1 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
Lane 2 : M1 (Mouse myeloblast myeloid leukemia cell line) whole cell lysate
Lane 3 : WEHI-231 (Mouse B cell lymphoma cell line) whole cell lysate
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 127 kDa
Observed band size: 170 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit (PMID: 10603370). It is exclusively restricted to cells of the myeloid lineages and monocytes, but not in T or B cell lines, so WEHI-231 cell lysate serves as a negative control (PMID: 2457584).
Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EPR19387] (ab184308)

Immunofluorescent analysis of 100% methanol-fixed RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) and WEHI-231 (Mouse B cell lymphoma cell line) cells labeling CD11b with ab184308 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1,000 dilution (green).

Confocal image showing membrane staining on RAW 264.7 cells and no staining on WEHI-231 cells (negative cell line).

The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1,000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed (ab150120) at 1/1,000 dilution (red).

The negative controls are as follows:
-ve control 1: ab184308 at 1/500 dilution followed by ab150120 at 1/1,000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1,000 dilution.
Immunoprecipitation - Anti-CD11b antibody [EPR19387] (ab184308)

CD11b was immunoprecipitated from 0.35 mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab184308 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184308 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: RAW 264.7 whole cell lysate, 10µg (Input).

Lane 2: ab184308 IP in RAW 264.7 whole cell lysate.

Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab184308 in RAW 264.7 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 8 seconds.
Anti-CD11b antibody [EPR19387] (ab184308)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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