Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD11b antibody [EPR19387] (ab184308)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19387] to CD11b
  • Suitable for: WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CD11b antibody [EPR19387]
    See all CD11b primary antibodies

  • Description

    Rabbit monoclonal [EPR19387] to CD11b

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: J774A.1, M1 and RAW 264.7 whole cell lysates. ICC/IF: RAW 264.7 cells. Flow Cyt: RAW 264.7 cells. IP: RAW 264.7 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-CD11b antibody [EPR19387] (ab184308)
    Flow Cytometry - Anti-CD11b antibody [EPR19387] (ab184308)

    Flow cytometric analysis of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling CD11b with ab184308 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] -Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-CD11b antibody [EPR19387] (ab184308)
    Western blot - Anti-CD11b antibody [EPR19387] (ab184308)
    All lanes : Anti-CD11b antibody [EPR19387] (ab184308) at 1/1000 dilution

    Lane 1 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
    Lane 2 : M1 (Mouse myeloblast myeloid leukemia cell line) whole cell lysate
    Lane 3 : WEHI-231 (Mouse B cell lymphoma cell line) whole cell lysate
    Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 127 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit (PMID: 10603370). It is exclusively restricted to cells of the myeloid lineages and monocytes, but not in T or B cell lines, so WEHI-231 cell lysate serves as a negative control (PMID: 2457584).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EPR19387] (ab184308)
    Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EPR19387] (ab184308)

    Immunofluorescent analysis of 100% methanol-fixed RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) and WEHI-231 (Mouse B cell lymphoma cell line) cells labeling CD11b with ab184308 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green).

    Confocal image showing membrane staining on RAW 264.7 cells and no staining on WEHI-231 cells (negative cell line).

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1,000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1,000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab184308 at 1/500 dilution followed by ab150120  at 1/1,000 dilution.
    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1,000 dilution.

  • Immunoprecipitation - Anti-CD11b antibody [EPR19387] (ab184308)
    Immunoprecipitation - Anti-CD11b antibody [EPR19387] (ab184308)

    CD11b was immunoprecipitated from 0.35 mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab184308 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184308 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: RAW 264.7 whole cell lysate, 10µg (Input).

    Lane 2: ab184308 IP in RAW 264.7 whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab184308 in RAW 264.7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

  • Anti-CD11b antibody [EPR19387] (ab184308)
    Anti-CD11b antibody [EPR19387] (ab184308)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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