Antibodies, Proteins, Kits and Reagents for Life Science

144 067 ₸ Product size

50 µg 144 067 ₸

Order now and get it on Wednesday February 24, 2021

PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)

Key features and details

PE/Cy5® Rat monoclonal [M1/70] to CD11b
Suitable for: Flow Cyt
Reacts with: Mouse
Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
Isotype: IgG2b

Product name

PE/Cy5® Anti-CD11b antibody [M1/70]
See all CD11b primary antibodies
Description

PE/Cy5® Rat monoclonal [M1/70] to CD11b
Host species

Rat
Conjugation

PE/Cy5®. Ex: 496nm, Em: 670nm
Tested applications

Suitable for: Flow Cytmore details
Species reactivity

Reacts with: Mouse
Immunogen

Tissue/ cell preparation (Mouse) - C57BL/10 mouse splenic T cells and concanavalin A activated C57BL/10 splenocytes.
Positive control
- Mouse spleen cells.
General notes

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purity

Affinity purified
Clonality

Monoclonal
Clone number

M1/70
Isotype

IgG2b
Light chain type

kappa
Research areas

Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)

Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling CD11b with ab25533 at 0.125 µg (solid line) or Rat IgG2b (PE/Cy5^®) isotype control at 0.125 µg (dashed line).
Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533) This image is courtesy of an anonymous Abreview

ab25533 staining CD11b in Human leukocytes by Flow Cytometry. Cells were prepared for staining after red blood cell lysis and were fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/50 in PBS/BSA 1%) for 30 minutes at 4°C. Gating strategy: Neutrophils.
See Abreview
Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)

Scatter plot showing mouse blood leukocyte cells stained with ab25533 (green). The cells were harvested from blood drawn from mice and prepared in PBS/BSA 1%. Cells were fixed in formaldehyde. The cells were then incubated in 1x PBS / 1% BSA to block non-specific protein-protein interactions followed by the antibody (1/100) for 30 min at 4ºC. Isotype control antibody (red) was used under the same conditions.
Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533) This image is courtesy of an anonymous Abreview

ab25533 staining CD11b in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
Gating Strategy: Live Macrophages.
See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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