Astana Biomed Group, an authorized Abcam distributor in Central Asia

PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)

Key features and details

  • PE/Cy5® Rat monoclonal [M1/70] to CD11b
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
  • Isotype: IgG2b

Overview

  • Product name

    PE/Cy5® Anti-CD11b antibody [M1/70]
    See all CD11b primary antibodies

  • Description

    PE/Cy5® Rat monoclonal [M1/70] to CD11b

  • Host species

    Rat

  • Conjugation

    PE/Cy5®. Ex: 496nm, Em: 670nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Tissue/ cell preparation (Mouse) - C57BL/10 mouse splenic T cells and concanavalin A activated C57BL/10 splenocytes.

  • Positive control

    • Mouse spleen cells.

  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

Images

  • Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
    Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)

    Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling CD11b with ab25533 at 0.125 µg (solid line) or Rat IgG2b (PE/Cy5®) isotype control at 0.125 µg (dashed line).

  • Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
    Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533) This image is courtesy of an anonymous Abreview
    ab25533 staining CD11b in Human leukocytes by Flow Cytometry. Cells were prepared for staining after red blood cell lysis and were fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/50 in PBS/BSA 1%) for 30 minutes at 4°C. Gating strategy: Neutrophils.

    See Abreview

  • Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
    Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
    Scatter plot showing mouse blood leukocyte cells stained with ab25533 (green). The cells were harvested from blood drawn from mice and prepared in PBS/BSA 1%. Cells were fixed in formaldehyde. The cells were then incubated in 1x PBS / 1% BSA to block non-specific protein-protein interactions followed by the antibody (1/100) for 30 min at 4ºC. Isotype control antibody (red) was used under the same conditions.
  • Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
    Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533) This image is courtesy of an anonymous Abreview
    ab25533 staining CD11b in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
    Gating Strategy: Live Macrophages.

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)

© 2021 Astana Biomed Group LLP. All rights reserved.