Anti-MLH1 antibody [EPR3893] (ab108622)
![Anti-MLH1 antibody [EPR3893] (ab108622)](https://www.abcam.com/ps/products/108/ab108622/Images/ab108622-386653-mlh1257172108622gdbanti-mlh1-antibody-epr3893-western-blot-human-lysate.jpg "Anti-MLH1 antibody [EPR3893] (ab108622)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3893] to MLH1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-MLH1 antibody [EPR3893]
See all MLH1 primary antibodies -
Description
Rabbit monoclonal [EPR3893] to MLH1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa, HAP1, Jurkat, 293, K562 and SH-SY5Y cell lysates.
-
General notes
To see more of the key markers and tools you need to study the hallmarks of cancer, including genome instability and mutation, please visit the following page.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3893 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-MLH1 antibody [EPR3893] (ab108622)All lanes : Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MLH1 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HCT116 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 85 kDaLanes 1-4: Merged signal (red and green). Green - ab108622 observed at 90 kDa. Red - loading control ab8245 observed at 37 kDa.
ab108622 Anti-MLH1 antibody [EPR3893] was shown to specifically react with MLH1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab267223 (knockout cell lysate ab257172) was used. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. ab108622 and Anti-GAPDH antibody [6C5] - Loading Control were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-MLH1 antibody [EPR3893] (ab108622)](https://www.abcam.com/ps/products/108/ab108622/Images/ab108622-256178-anti-mlh1-antibody-epr3893-western-blot.jpg)
Western blot - Anti-MLH1 antibody [EPR3893] (ab108622)All lanes : Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : MLH1 knockout HAP1 cell lysate
Lane 3 : HCT116 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 85 kDaLanes 1 - 4: Merged signal (red and green). Green - ab108622 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108622 was shown to recognize MLH1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when MLH1 knockout samples were examined. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. ab108622 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-MLH1 antibody [EPR3893] (ab108622)](https://www.abcam.com/ps/products/108/ab108622/Images/ab108622-121359-anti-mlh1-antibody-epr3893-western-blot.jpg)
Western blot - Anti-MLH1 antibody [EPR3893] (ab108622)All lanes : Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution
Lane 1 : 293 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 85 kDa
-
![Western blot - Anti-MLH1 antibody [EPR3893] (ab108622)](https://www.abcam.com/ps/products/108/ab108622/Images/ab108622-249767-anti-mlh1-antibody-epr3893-western-blot.jpg)
Western blot - Anti-MLH1 antibody [EPR3893] (ab108622) This image is courtesy of an anonymous abreview.All lanes : Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution
Lane 1 : Hela Cells Whole Cell Lysates
Lane 2 : HCT116 Whole Cell Lysates
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Polyclonal to Rabbit IgG (HRP) at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Exposure time: 30 seconds
-
![Anti-MLH1 antibody [EPR3893] (ab108622)](https://www.abcam.com/ps/products/108/ab108622/Images/ab108622-5-benefits-of-recombinant-antibodies.png)
Anti-MLH1 antibody [EPR3893] (ab108622)
