Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3893] to MLH1
Suitable for: WB
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-MLH1 antibody [EPR3893]
See all MLH1 primary antibodies
Description

Rabbit monoclonal [EPR3893] to MLH1
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HAP1, Jurkat, 293, K562 and SH-SY5Y cell lysates.
General notes
To see more of the key markers and tools you need to study the hallmarks of cancer, including genome instability and mutation, please visit the following page.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3893
Isotype

IgG
Research areas

Western blot - Anti-MLH1 antibody [EPR3893] (ab108622)

All lanes : Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MLH1 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HCT116 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 85 kDa
Observed band size: 85 kDa

Lanes 1-4: Merged signal (red and green). Green - ab108622 observed at 90 kDa. Red - loading control ab8245 observed at 37 kDa.

ab108622 Anti-MLH1 antibody [EPR3893] was shown to specifically react with MLH1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab267223 (knockout cell lysate ab257172) was used. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. ab108622 and Anti-GAPDH antibody [6C5] - Loading Control were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-MLH1 antibody [EPR3893] (ab108622)

All lanes : Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : MLH1 knockout HAP1 cell lysate
Lane 3 : HCT116 cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 85 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab108622 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab108622 was shown to recognize MLH1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when MLH1 knockout samples were examined. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. ab108622 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-MLH1 antibody [EPR3893] (ab108622)

All lanes : Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution

Lane 1 : 293 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : SH-SY5Y cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 85 kDa
Western blot - Anti-MLH1 antibody [EPR3893] (ab108622) This image is courtesy of an anonymous abreview.

All lanes : Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution

Lane 1 : Hela Cells Whole Cell Lysates
Lane 2 : HCT116 Whole Cell Lysates

Lysates/proteins at 30 µg per lane.

Secondary
All lanes : Goat Polyclonal to Rabbit IgG (HRP) at 1/2000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 85 kDa

Exposure time: 30 seconds
See Abreview
Anti-MLH1 antibody [EPR3893] (ab108622)

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