Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)
![Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)](https://www.abcam.com/ps/products/250/ab250761/Images/ab250761-2-anti-mad1-antibody-epr14676-23-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14676-23] to MAD1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free
See all MAD1 primary antibodies -
Description
Rabbit monoclonal [EPR14676-23] to MAD1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250761 is the carrier-free version of ab184560. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250761 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR14676-23 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)This data was developed using ab184560, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin fixed and paraffin embedded Human melanoma tissue labeling MAD1 with ab184560 at 1/50 dilution, counterstained with Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)](https://www.abcam.com/ps/products/250/ab250761/Images/ab250761-398272-anti-mad1-antibody-epr14676-23-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)All lanes : Anti-MAD1 antibody [EPR14676-23] (ab184560) at 1/20000 dilution
Lane 1 : HepG2 lysate
Lane 2 : A431 lysate
Lane 3 : HeLa lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 83 kDaThis data was developed using ab184560, the same antibody clone in a different buffer formulation.
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![Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)](https://www.abcam.com/ps/products/250/ab250761/Images/ab250761-3-benefits-of-recombinant-antibodies.png)
Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)