Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3730(2)] to PAX5 - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB, IHC-P, Flow Cyt, ChIP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free
    See all PAX5 primary antibodies

  • Description

    Rabbit monoclonal [EPR3730(2)] to PAX5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Ramos and Daudi cell lysates and human tonsil and mouse spleen tissue lysates. IHC-P: Human tonsil, hodgkin's lymphoma, spleen and diffuse B-cell lymphoma tissues. ICC/IF: Ramos cells. Flow Cyt: Ramos cells. IP: Mouse spleen tissue lysate and Ramos cells. ChIP: Ramos cells

  • General notes

    Ab193556 is the carrier-free version of ab109443. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab193556 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labelling PAX5 with purified ab109443 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109443).

  • Immunocytochemistry/ Immunofluorescence - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    Immunocytochemistry/ Immunofluorescence - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

    Immunocytochemistry/Immunofluorescence analysis of Ramos cells labelling PAX5 with purified ab109443 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109443).

  • Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

    ab109443 (purified) at 1/20 dilution immunoprecipitating PAX5 in mouse spleen lysate 10 µg.
    Lane 1 (input): mouse spleen lysate 10 µg
    Lane 2 (+): ab109443 & mouse spleen lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109443 in mouse spleen lysate

    For western blotting, ab109443 at 1/500 dilution (0.268 µg/mL) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution was used.

    Blocking and diluting buffer: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109443)

  • ChIP - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    ChIP - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

    Chromatin was prepared from Ramos cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 µg of chromatin, 5 µg of abzz (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 25 µl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are from paper PMID:19806612
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109443).

  • Flow Cytometry - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    Flow Cytometry - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

    Flow Cytometry analysis of Ramos cells labelling PAX5 with purified ab109443 at 1/30 (red). Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109443).

  • Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

    ab109443 (purified) at 1/20 dilution immunoprecipitating PAX5 in Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg.

    Lane 1 (input): Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg
    Lane 2 (+): ab109443 & Ramos whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109443 in Ramos whole cell lysate

    For western blotting, ab109443 at 1/500 dilution (0.268 µg/mL) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution was used.

    Blocking and diluting buffer: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109443)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PAX5 with purified ab109443 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109443).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human B-cell lymphoma tissue labelling PAX5 with purified ab109443 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109443).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PAX5 with unpurified ab109443.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109443).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Hodgkin's lymphoma tissue labelling PAX5 with unpurified ab109443.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109443).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

    ab109443 showing negative staining in Normal kidney tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109443).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal spleen tissue labelling PAX5 with unpurified ab109443.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109443).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human diffuse B-cell lymphoma tissue labelling PAX5 with unpurified ab109443.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109443).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    Flow Cytometry - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

    Overlay histogram showing Ramos cells stained with unpurified ab109443 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab109443, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109443).

  • OI-RD Scanning - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    OI-RD Scanning - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109443).

  • Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
    Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

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