Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP34] to PAX5 - BSA and Azide free
  • Suitable for: IP, ChIP, Flow Cyt, IHC-P, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-PAX5 antibody [SP34] - BSA and Azide free
    See all PAX5 primary antibodies

  • Description

    Rabbit monoclonal [SP34] to PAX5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ChIP, Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Ramos cells. IHC-P: Human tonsil and B cell lymphoma and rat spleen tissues. IP: Ramos whole cell lysates. WB: Ramos, Daudi, Jurkat and U-937 cell lysates.

  • General notes

    ab237773 is the carrier-free version of ab227635.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling PAX5 with ab227635 at 1/50 dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab228136 for 30 mins at room temperature.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227737).

  • ChIP - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)
    ChIP - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

    Chromatin was prepared from Ramos cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab227635 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
    Primers and probes are commercial primers from Paper (PMID: 19806612)
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab227635).

  • Immunoprecipitation - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)
    Immunoprecipitation - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

    ab227635 (purified) at 1/20 dilution immunoprecipitating PAX5 in Ramos whole cell lysate.

    Lane 1 (input): Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab227635 & Ramos whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab227635 in Ramos whole cell lysate

    For western blotting, ab227648 (purified at 1/500 dilution (0.344 µg/ml) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution were used.

    Blocking and diluting buffer: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab227635).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling PAX5 with ab227635 at 1/50 dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.

    The section was incubated with ab228136 for 30 mins at room temperature.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227737).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

    Immunohistochemical analysis of human tonsil tissue labeling PAX5 using ab227635.

    This data was developed using the same antibody clone in a different buffer formulation (ab227635).

  • Flow Cytometry - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)
    Flow Cytometry - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

    Flow Cytometry analysis of Ramos(Human Burkitt's lymphoma B lymphocyte) cells labeling PAX5 with purified ab227635 at 1:20 dilution (8.6 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227737).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

    Immunohistochemical analysis of Human B cell lymphoma tissue staining PAX5 using ab227635. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227635).

  • Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)
    Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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