Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-H-protein antibody [OTI3G1] (ab156763)

Key features and details

  • Mouse monoclonal [OTI3G1] to H-protein
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human, African green monkey
  • Isotype: IgG2a

Overview

  • Product name

    Anti-H-protein antibody [OTI3G1]

  • Description

    Mouse monoclonal [OTI3G1] to H-protein

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human, African green monkey

  • Immunogen

    Recombinant full length protein corresponding to Human H-protein aa 1-477. H-protein (NP_004988) produced in HEK-293T cell.
    Sequence:

    MMEKNTSEGPACSPEETASESAKVPTAEPPGEVAVSESTREEQVPKPQAP APQAPTASTATKPAPPSEDVPSAPLLLTLDDVSSSSVTVSWEPPERLGRL GLQGYVLELCREGASEWVPVSARPMMVTQQTVRNLALGDKFLLRVSAVSS AGAGPPAMLDQPIHIRENIEAPKIRVPRHLRQTYIRQVGETVNLQIPFQG KPKPQATWTHNGHALDSQRVSMRTGDQDSILFIRSAQRSDSGRYELTVRV EDLEAKAVIDILVIEKPGPPSSIRLLDVWGCNAALQWTPPQDTGNTELLG YMVQKADKKTGQWFTVLERYHPTTCTISDLIIGNSYSFRVFSENLCGLST SATVTKELAHIQKADIAAKPKGFIERDFSEAPSFTQPLADHTSTPGYSTQ LFCSVRASPKPKIIWMKNKMEIQGNPKYRALSEQGVCTLEIRKPSPFDSG VYTCKAINVLGEASVDCRLEVKASAAH


    Database link: Q13203
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human colon adenocarcinoma tissue, Human lung carcinoma tissue and Human pancreas tissue. HeLa cells.

  • General notes

    The clone number has been updated from 3G1 to OTI3G1, both clone numbers name the same clone.

    Previously labelled as MYBPH.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    OTI3G1

  • Isotype

    IgG2a

  • Research areas

Images

  • Western blot - Anti-H-protein antibody [OTI3G1] (ab156763)
    Western blot - Anti-H-protein antibody [OTI3G1] (ab156763)
    All lanes : Anti-H-protein antibody [OTI3G1] (ab156763) at 1/4000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY H-protein

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 52 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-H-protein antibody [OTI3G1] (ab156763)
    Immunocytochemistry/ Immunofluorescence - Anti-H-protein antibody [OTI3G1] (ab156763)

    Immunofluorescence analysis of COS7 cells transiently transfected by pCMV6-ENTRY H-protein labeling H-protein with ab156763 at 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-protein antibody [OTI3G1] (ab156763)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-protein antibody [OTI3G1] (ab156763)

    Paraffin embedded Human colon adenocarcinoma tissue labeling H-protein with ab156763 at 1/150 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-protein antibody [OTI3G1] (ab156763)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-protein antibody [OTI3G1] (ab156763)

    Paraffin embedded Human lung carcinoma tissue labeling H-protein with ab156763 at 1/150 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-protein antibody [OTI3G1] (ab156763)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-protein antibody [OTI3G1] (ab156763)

    Paraffin embedded Human pancreas tissue labeling H-protein with ab156763 at 1/150 in immunohistochemical analysis.

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