Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HMG4 antibody [EP2839Y] (ab75782)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2839Y] to HMG4
  • Suitable for: ICC, IP, Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HMG4 antibody [EP2839Y]
    See all HMG4 primary antibodies

  • Description

    Rabbit monoclonal [EP2839Y] to HMG4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HeLa whole cell lysate; Flow Cyt: HeLa cells; ICC: 293T; IHC: Rat and mouse liver tissue, Human placenta tissue; WB: HeLa whole cell lysate. Human bladder and placenta tissue lysates. Mouse brain and bladder tissue lysates. Rat brain, bladder, and placenta tissue lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-HMG4 antibody [EP2839Y] (ab75782)
    Immunoprecipitation - Anti-HMG4 antibody [EP2839Y] (ab75782)

    Purified ab75782 at 1:20 dilution (0.5µg) immunoprecipitating HMG4 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab75782 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75782 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP)(ab131366) (1:1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 25 kDa

  • Immunohistochemistry paraffin embedded sections - Anti-HMG4 antibody [EP2839Y] (ab75782)
    Immunohistochemistry paraffin embedded sections - Anti-HMG4 antibody [EP2839Y] (ab75782)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling HMG4 with Purified ab75782 at 1:100 dilution (1.13 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry - Anti-HMG4 antibody [EP2839Y] (ab75782)
    Immunocytochemistry - Anti-HMG4 antibody [EP2839Y] (ab75782)

    Immunocytochemistry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling HMG4 with Purified ab75782 at 1:250 dilution (0.452 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry paraffin embedded sections - Anti-HMG4 antibody [EP2839Y] (ab75782)
    Immunohistochemistry paraffin embedded sections - Anti-HMG4 antibody [EP2839Y] (ab75782)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling HMG4 with Purified ab75782 at 1:100 dilution (1.13 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry - Anti-HMG4 antibody [EP2839Y] (ab75782)
    Flow Cytometry - Anti-HMG4 antibody [EP2839Y] (ab75782)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling HMG4 with Purified ab75782 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry paraffin embedded sections - Anti-HMG4 antibody [EP2839Y] (ab75782)
    Immunohistochemistry paraffin embedded sections - Anti-HMG4 antibody [EP2839Y] (ab75782)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue sections labeling HMG4 with Purified ab75782 at 1:100 dilution (1.13 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-HMG4 antibody [EP2839Y] (ab75782)
    Western blot - Anti-HMG4 antibody [EP2839Y] (ab75782)
    Anti-HMG4 antibody [EP2839Y] (ab75782) at 1/5000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 23 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

  • Western blot - Anti-HMG4 antibody [EP2839Y] (ab75782)
    Western blot - Anti-HMG4 antibody [EP2839Y] (ab75782)
    All lanes : Anti-HMG4 antibody [EP2839Y] (ab75782) at 1/1000 dilution (Purified)

    Lane 1 : Human bladder lysate
    Lane 2 : Human placenta lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Mouse bladder lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat bladder lysate
    Lane 7 : Rat placenta lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 23 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

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