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Epigenetics and Nuclear Signaling Chromatin Binding Proteins DNA / RNA binding

Anti-HMG4 antibody (ab72544)

Anti-HMG4 antibody (ab72544)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to HMG4
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-HMG4 antibody
    See all HMG4 primary antibodies
  • Description

    Rabbit polyclonal to HMG4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 125 and 175 of human HMG4 (NP_005333.2).

  • Positive control

    • HeLa and 293T whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Chromatin assembly

Images

  • Western blot - Anti-HMG4 antibody (ab72544)
    Western blot - Anti-HMG4 antibody (ab72544)
    All lanes : Anti-HMG4 antibody (ab72544) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 23 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody (ab72544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody (ab72544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling HMG4 with ab72544 at 1/200 (1µg/ml). Detection: DAB.
  • Immunoprecipitation - Anti-HMG4 antibody (ab72544)
    Immunoprecipitation - Anti-HMG4 antibody (ab72544)
    Detection of Human HMG4 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72544 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: Flow Cyt, ICC, IHC-P, IP, WB

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