Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] - BSA and Azide free (ab248793)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8385] to Amino-terminal enhancer of split/AES - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] - BSA and Azide free
    See all Amino-terminal enhancer of split/AES primary antibodies

  • Description

    Rabbit monoclonal [EPR8385] to Amino-terminal enhancer of split/AES - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WBmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HCT116, 293T, HeLa, PC3 and Jurkat cell lysates. ICC: HeLa cells.

  • General notes

    ab248793 is the carrier-free version of ab137060 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248793 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Amino-terminal enhancer of split

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] - BSA and Azide free (ab248793)
    Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] - BSA and Azide free (ab248793)
    All lanes : Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060) at 1/1000 dilution

    Lane 1 : Wild-type HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
    Lane 2 : AES knockout HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
    Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 4 : PC3 (Human prostate adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    This data was developed using ab137060, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab137060 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab137060 Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] was shown to specifically react with Amino-terminal enhancer of split/AES in wild-type HCT cells. Loss of signal was observed when knockout cell line ab266886 (knockout cell lysate ab257818) was used. Wild-type and Amino-terminal enhancer of split/AES knockout samples were subjected to SDS-PAGE. ab137060 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] - BSA and Azide free (ab248793)
    Immunocytochemistry - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] - BSA and Azide free (ab248793)
    This data was developed using ab137060, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells labelling Amino-terminal enhancer of split/AES using ab137060 at 1/250 dilution.
  • Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] - BSA and Azide free (ab248793)
    Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] - BSA and Azide free (ab248793)
    All lanes : Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : PC3 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 21 kDa



    This data was developed using ab137060, the same antibody clone in a different buffer formulation.

  • Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] - BSA and Azide free (ab248793)
    Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] - BSA and Azide free (ab248793)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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