Anti-Hsp20 antibody (ab13491)
Key features and details
- Rabbit polyclonal to Hsp20
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Hsp20 antibody
See all Hsp20 primary antibodies -
Description
Rabbit polyclonal to Hsp20 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Rat
-
Immunogen
Rat skeletal muscle Hsp20.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.09% Sodium azide
Constituents: 0.268% PBS, 3% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Hsp20 antibody (ab13491)Anti-Hsp20 antibody (ab13491) at 1/2000 dilution + Lysates prepared from rat heart tissue
-
Immunocytochemistry/ Immunofluorescence - Anti-Hsp20 antibody (ab13491)ICC/IF image of ab13491 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13491, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
