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Anti-HSPA4 antibody [EPR14166] (ab185962)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14166] to HSPA4
  • Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HSPA4 antibody [EPR14166]
    See all HSPA4 primary antibodies

  • Description

    Rabbit monoclonal [EPR14166] to HSPA4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa and HepG2 cells and cell lysates; K562, Human cerebellum, Mouse heart, Rat brain and Rat heart lysates; Human brain tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HSPA4 antibody [EPR14166] (ab185962)
    Western blot - Anti-HSPA4 antibody [EPR14166] (ab185962)
    All lanes : Anti-HSPA4 antibody [EPR14166] (ab185962) at 1/10000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 94 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HSPA4 antibody [EPR14166] (ab185962)
    Immunocytochemistry/ Immunofluorescence - Anti-HSPA4 antibody [EPR14166] (ab185962)

    Immunofluorescence analysis of -20°C acetone-fixed HeLa cells labeling HSPA4 with ab185962 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).

  • Western blot - Anti-HSPA4 antibody [EPR14166] (ab185962)
    Western blot - Anti-HSPA4 antibody [EPR14166] (ab185962)
    All lanes : Anti-HSPA4 antibody [EPR14166] (ab185962) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 94 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA4 antibody [EPR14166] (ab185962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA4 antibody [EPR14166] (ab185962)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling HSPA4 with ab185962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-HSPA4 antibody [EPR14166] (ab185962)
    Immunocytochemistry/ Immunofluorescence - Anti-HSPA4 antibody [EPR14166] (ab185962)

    Immunofluorescence analysis of 4% paraformaldehyde-fixed HepG2 cells labeling HSPA4 with ab185962 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).

  • Flow Cytometry - Anti-HSPA4 antibody [EPR14166] (ab185962)
    Flow Cytometry - Anti-HSPA4 antibody [EPR14166] (ab185962)

    Flow Cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling HSPA4 with ab185962 at 1/200 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Anti-HSPA4 antibody [EPR14166] (ab185962)
    Anti-HSPA4 antibody [EPR14166] (ab185962)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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