Recombinant human Hsp90 beta protein (Active) (ab80033)
Key features and details
- Expression system: Baculovirus infected BTI-TN-5B1-4 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, ELISA, Inhibition Assay, Functional Studies, SDS-PAGE
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Product name
Recombinant human Hsp90 beta protein (Active)
See all Hsp90 beta proteins and peptides -
Biological activity
1. The reactions are in 33 mM Hepes pH7.2, 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µl reaction at 37ºC. It is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.
2. The protein concentrations (where applicable) were 2 µM Hsp90 (Hsp90 beta), 2 µM Ahsa1 (His tagged human Ahsa1), 4 µM p23 (cleaved human p23), 4 µM HOP (His-tagged human HOP).
3. Addition of a two-fold excess of p23 reduced Aha1-mediated Hsp90 stimulation by 30% (when using 2 µM Hsp90 and Aha1).
4. Addition of a two -fold excess of HOP completely eliminated Aha1-mediated ATPase stimulation as well as intrinsic Hsp90 ATPase activity.
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Purity
> 90 % SDS-PAGE.
ab80033 is Affinity Purified, Endotoxin-free -
Expression system
Baculovirus infected BTI-TN-5B1-4 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDA LDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLIN NLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHN DDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKE VVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKI EDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEE YGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKK NNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKI LKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRR LSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFV ERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEK KKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTA NMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKA VKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEP NAAVPDEIPPLEGDEDASRMEEVD -
Amino acids
1 to 724
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Constituents: 0.0154% DTT, 0.242% Tris, 0.00292% EDTA, 10% Glycerol (glycerin, glycerine), 1.015% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Western blot - Recombinant human Hsp90 beta protein (ab80033)
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SDS-PAGE - Recombinant human Hsp90 beta protein (Active) (ab80033)SDS-PAGE of Hsp90 beta protein (ab80033).
