Anti-PP-T antibody (ab26053)
Key features and details
- Rabbit polyclonal to PP-T
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PP-T antibody
See all PP-T primary antibodies -
Description
Rabbit polyclonal to PP-T -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
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Positive control
- Recombinant Human PP-T protein (ab91714) can be used as a positive control in WB. This antibody gave a positive result in the following lysates: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Brain (Mouse) Tissue Lysate Testis (Mouse) Tissue Lysate - normal tissue Brain (Rat) Tissue Lysate - normal tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanAll applications DogXenopus laevisApplication Abreviews Notes WB (1) Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 57 kDa).Notes WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 57 kDa).Target
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Function
May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.
Contains 3 TPR repeats. -
Cellular localization
Nucleus. Cytoplasm. Predominantly nuclear. But also present in the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5536 Human
- Entrez Gene: 19060 Mouse
- Entrez Gene: 65179 Rat
- Omim: 600658 Human
- SwissProt: P53041 Human
- SwissProt: Q60676 Mouse
- SwissProt: P53042 Rat
- Unigene: 654604 Human
see all -
Alternative names
- FLJ36922 antibody
- FLJ55954 antibody
- OTTHUMP00000165834 antibody
see all
Images
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Western blot - Anti-PP-T antibody (ab26053)All lanes : Anti-PP-T antibody (ab26053) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted? -
Western blot - Anti-PP-T antibody (ab26053)All lanes : Anti-PP-T antibody (ab26053) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 3 : Brain (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Datasheets and documents
References (2)
ab26053 has been referenced in 2 publications.
- Norris-Mullins B et al. Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major. Parasite 21:25 (2014). WB . PubMed: 24890370
- Zhang Y et al. Estrogen inhibits glucocorticoid action via protein phosphatase 5 (PP5)-mediated glucocorticoid receptor dephosphorylation. J Biol Chem 284:24542-52 (2009). WB ; Human . PubMed: 19586900
Images
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Western blot - Anti-PP-T antibody (ab26053)All lanes : Anti-PP-T antibody (ab26053) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted? -
Western blot - Anti-PP-T antibody (ab26053)All lanes : Anti-PP-T antibody (ab26053) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 3 : Brain (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
