Anti-PP-T antibody [2E11] (ab223355)
![Anti-PP-T antibody [2E11] (ab223355)](https://www.abcam.com/ps/products/223/ab223355/Images/ab223355-283013-ab223355ICC1.png "Anti-PP-T antibody [2E11] (ab223355)")
Key features and details
- Mouse monoclonal [2E11] to PP-T
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-PP-T antibody [2E11]
See all PP-T primary antibodies -
Description
Mouse monoclonal [2E11] to PP-T -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant full length protein corresponding to Human PP-T aa 1 to the C-terminus.
Database link: P53041 -
Positive control
- WB: A431, Jurkat and HEK-293 cell lysates. ICC/IF: HEK-293 and HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2E11 -
Isotype
IgG2a -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PP-T antibody [2E11] (ab223355)
2% formaldehyde-fixed HEK-293 (human epithelial cell line from embryonic kidney) cells stained for PP-T (green) using ab223355 at 1/50 dilution in ICC/IF. Secondary: Goat Anti-Mouse Alexa Fluor 488 at 1/100 dilution for 1 hour at RT. Counterstain: DAPI (blue) nuclear stain.
(A) DAPI nuclear stain (B) ab223355 (C) Merge.
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![Western blot - Anti-PP-T antibody [2E11] (ab223355)](https://www.abcam.com/ps/products/223/ab223355/Images/ab223355-283012-223355WBj.jpg)
Western blot - Anti-PP-T antibody [2E11] (ab223355)All lanes : Anti-PP-T antibody [2E11] (ab223355) at 1/500 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 56.9 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutesBlocking buffer: 5% skim milk.
Primary and secondary antibodies were incubated for 1 hour at RT.
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![Immunocytochemistry/ Immunofluorescence - Anti-PP-T antibody [2E11] (ab223355)](https://www.abcam.com/ps/products/223/ab223355/Images/ab223355-283014-ab223355ICC2.png)
Immunocytochemistry/ Immunofluorescence - Anti-PP-T antibody [2E11] (ab223355)4% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PP-T (green) using ab223355 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Mouse ATTO 488 at 1/100 dilution for 1 hour at RT. Counterstain: DAPI (blue) nuclear stain at 1/5000 for 5 min RT.
(A) DAPI nuclear stain (B) ab223355 (C) Merge.
