Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [2E11] to PP-T
Suitable for: ICC/IF, WB
Reacts with: Human
Isotype: IgG2a

Product name

Anti-PP-T antibody [2E11]
See all PP-T primary antibodies
Description

Mouse monoclonal [2E11] to PP-T
Host species

Mouse
Tested applications

Suitable for: ICC/IF, WBmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen

Recombinant full length protein corresponding to Human PP-T aa 1 to the C-terminus.
Database link: P53041

Run BLAST with Run BLAST with
Positive control
- WB: A431, Jurkat and HEK-293 cell lysates. ICC/IF: HEK-293 and HeLa cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

2E11
Isotype

IgG2a
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-PP-T antibody [2E11] (ab223355)

2% formaldehyde-fixed HEK-293 (human epithelial cell line from embryonic kidney) cells stained for PP-T (green) using ab223355 at 1/50 dilution in ICC/IF. Secondary: Goat Anti-Mouse Alexa Fluor 488 at 1/100 dilution for 1 hour at RT. Counterstain: DAPI (blue) nuclear stain.

(A) DAPI nuclear stain (B) ab223355 (C) Merge.
Western blot - Anti-PP-T antibody [2E11] (ab223355)

All lanes : Anti-PP-T antibody [2E11] (ab223355) at 1/500 dilution

Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Mouse IgG HRP at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 56.9 kDa
Observed band size: 58 kDa
why is the actual band size different from the predicted?

Exposure time: 6 minutes

Blocking buffer: 5% skim milk.

Primary and secondary antibodies were incubated for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence - Anti-PP-T antibody [2E11] (ab223355)

4% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PP-T (green) using ab223355 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Mouse ATTO 488 at 1/100 dilution for 1 hour at RT. Counterstain: DAPI (blue) nuclear stain at 1/5000 for 5 min RT.

(A) DAPI nuclear stain (B) ab223355 (C) Merge.

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