Antibodies, Proteins, Kits and Reagents for Life Science

Anti-PTP1B antibody [OTI1D10] (ab124375)

Key features and details

Mouse monoclonal [OTI1D10] to PTP1B
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Dog, Human, African green monkey
Isotype: IgG2a

Product name

Anti-PTP1B antibody [OTI1D10]
See all PTP1B primary antibodies
Description

Mouse monoclonal [OTI1D10] to PTP1B
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	African green monkey
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human PTP1B.
Database link: NP_002818
Positive control
- WB: PTP1B-transfected HEK293T, HepG2, HeLa, COS7, Jurkat, MDCK, PC12, and MCF7 cell lysates IHC-P: Human colon, Human lymph node and Human lymphoma tissuesICC/IF: COS7 cells transiently transfected by PTP1BFlow Cyt: PTP1B-transfected HEK293T cells
General notes

The clone number has been updated from 1D10 to OTI1D10, both clone numbers name the same clone.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography
Clonality

Monoclonal
Clone number

OTI1D10
Isotype

IgG2a
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (ab124375)

Staining of PTP1B in Paraffin-embedded Human colon tissue by Immunohistochemistry using ab124375 at a dilution of 1/150.
Western blot - Anti-PTP1B antibody [OTI1D10] (ab124375)

All lanes : Anti-PTP1B antibody [OTI1D10] (ab124375)

Lane 1 : Wild-type HeLa cells
Lane 2 : PTPB1 knockout HeLa cells

Lysates/proteins at 10 µg per lane.

Predicted band size: 50 kDa
Western blot - Anti-PTP1B antibody [OTI1D10] (ab124375)

All lanes : Anti-PTP1B antibody [OTI1D10] (ab124375) at 1/500 dilution

Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PTP1B

Lysates/proteins at 5 µg per lane.

Predicted band size: 50 kDa
Western blot - Anti-PTP1B antibody [OTI1D10] (ab124375)

All lanes : Anti-PTP1B antibody [OTI1D10] (ab124375) at 1/500 dilution

Lane 1 : HepG2 cell extract
Lane 2 : HeLa cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 cell extract
Lane 5 : COS7 cell extract
Lane 6 : Jurkat cell extract
Lane 7 : MDCK cell extract
Lane 8 : PC12 cell extract
Lane 9 : MCF7 cell extract

Lysates/proteins at 35 µg per lane.

Predicted band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (ab124375)

Staining of PTP1B in Paraffin-embedded Human lymph node tissue by Immunohistochemistry using ab124375 at a dilution of 1/150.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (ab124375)

Staining of PTP1B in Paraffin-embedded Human lymphoma tissue by Immunohistochemistry using ab124375 at a dilution of 1/150.
Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [OTI1D10] (ab124375)

Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTP1B using ab124375 at a dilution of 1/100.
Flow Cytometry - Anti-PTP1B antibody [OTI1D10] (ab124375)

HEK293T cells transfected with either PTP1B overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab124375 at a dilution of 1/100, and then analyzed by flow cytometry.

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