Anti-PTP1B antibody [EPR1836(2)] - BSA and Azide free (ab232050)
![Anti-PTP1B antibody [EPR1836(2)] - BSA and Azide free (ab232050)](https://www.abcam.com/ps/products/232/ab232050/Images/ab232050-308912-anti-ptp1b-antibody-epr18362-western-blot.jpg "Anti-PTP1B antibody [EPR1836(2)] - BSA and Azide free (ab232050)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1836(2)] to PTP1B - BSA and Azide free
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-PTP1B antibody [EPR1836(2)] - BSA and Azide free
See all PTP1B primary antibodies -
Description
Rabbit monoclonal [EPR1836(2)] to PTP1B - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HAP1 cell lysate; Jurkat and MCF7 cell lysate.
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General notes
ab232050 is the carrier-free version of ab133244 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab232050 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1836(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PTP1B antibody [EPR1836(2)] - BSA and Azide free (ab232050)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PTP1B knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133244 observed at 54 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133244 was shown to recognize PTP1B when PTP1B knockout samples were used, along with additional cross-reactive bands. Wild-type and PTP1B knockout samples were subjected to SDS-PAGE. ab133244 and ab8245 (loading control to GAPDH) were diluted 1/1,000 and 1/2,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133244).
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![Anti-PTP1B antibody [EPR1836(2)] - BSA and Azide free (ab232050)](https://www.abcam.com/ps/products/232/ab232050/Images/ab232050-2-benefits-of-recombinant-antibodies.png)
Anti-PTP1B antibody [EPR1836(2)] - BSA and Azide free (ab232050)
