Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22474] to PTP1B - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PTP1B antibody [EPR22474] - BSA and Azide free
    See all PTP1B primary antibodies

  • Description

    Rabbit monoclonal [EPR22474] to PTP1B - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    IHC is recommended for human only.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate. IHC-P: Human breast cancer and colon cancer tissue. ICC/IF: HeLa, HAP1 and HCT 116 cells. Flow Cyt: HeLa and HCT 116 cells. IP: PTP1B IP in HCT 116 whole cell lysate.

  • General notes

    Ab245984 is the carrier-free version of ab244207. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab245984 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)
    Western blot - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)
    All lanes : Anti-PTP1B antibody [EPR22474] (ab244207) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PTPN1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab244207).

      Lanes 1- 2: Merged signal (red and green). Green - ab244207 observed at 50 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab244207 was shown to react with PTP1B in wild-type HeLa cells in western blot. The band observed in knockout cell line ab265014 (knockout cell lysate ab257617) lane below 50kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and PTPN1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab244207 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling PTP1B with ab244207 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human colon cancer (PMID:27752061) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244207).
  • Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)
    Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PTP1B with ab244207 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in HeLa cells. The nuclear counterstain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244207).
  • Flow Cytometry - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)
    Flow Cytometry - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling PTP1B with ab244207 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244207).
  • Immunoprecipitation - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)
    Immunoprecipitation - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)

    PTP1B  was immunoprecipitated from 0.35 mg HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate with ab244207 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab244207 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
    Lane 1: HCT 116 whole cell lysate 10 µg (Input).
    Lane 2: ab244207 IP in HCT 116 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab244207 in HCT 116 whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

     

    The observed MW is consistent with what described in the literatures. (PMID: 18253097; PMID: 11895943; PMID: 19797268).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244207).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling PTP1B with ab244207 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Higher cytoplasmic expression in human breast cancer than that of adjacent normal tissues (PMID: 27465552) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244207).
  • Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)
    Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Wild type and PTP1B-knockout HAP1 (Human chronic myelogenous leukemia near-haploid cell line) cells labeling PTP1B with ab244207 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing no staining in PTP1B-knockout HAP1 cells. The nuclear counterstain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244207).
  • Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)
    Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cells labeling PTP1B with ab244207 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in HCT 116 cells. The nuclear counterstain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244207).
  • Flow Cytometry - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)
    Flow Cytometry - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cell line labeling PTP1B with ab244207 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244207).
  • Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)
    Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PTP1B antibody [EPR22474] - BSA and Azide free (ab245984)

© 2021 Astana Biomed Group LLP. All rights reserved.