HRP Anti-PTP1B antibody [EP1841Y] (ab207635)
![HRP Anti-PTP1B antibody [EP1841Y] (ab207635)](https://www.abcam.com/ps/products/207/ab207635/Images/ab207635-265708-anti-ptp1b-antibody-ep1841y-hrp-western-blot.jpg "HRP Anti-PTP1B antibody [EP1841Y] (ab207635)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1841Y] to PTP1B
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-PTP1B antibody [EP1841Y]
See all PTP1B primary antibodies -
Description
HRP Rabbit monoclonal [EP1841Y] to PTP1B -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HepG2, Jurkat and wildtype HAP1 whole cell lysates.
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General notes
Alternative versions available:
Anti-PTP1B antibody [EP1841Y] (ab75856) - Knockout validatedOur RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP1841Y -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-PTP1B antibody [EP1841Y] (ab207635)All lanes : HRP Anti-PTP1B antibody [EP1841Y] (ab207635) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 4 : Wild-type HAP1 cell lysate at 20 µg
Lane 5 : PTPB1 knockout HAP1 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab207635 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-PTP1B antibody [EP1841Y] (ab207635)](https://www.abcam.com/ps/products/207/ab207635/Images/ab207635-2-benefits-of-recombinant-antibodies.png)
HRP Anti-PTP1B antibody [EP1841Y] (ab207635)
