Anti-PTP1B antibody [EPR1836(2)] (ab133244)
![Anti-PTP1B antibody [EPR1836(2)] (ab133244)](https://www.abcam.com/ps/products/133/ab133244/Images/ab133244-255515-anti-ptp1b-antibody-epr18362-western-blot.jpg "Anti-PTP1B antibody [EPR1836(2)] (ab133244)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1836(2)] to PTP1B
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-PTP1B antibody [EPR1836(2)]
See all PTP1B primary antibodies -
Description
Rabbit monoclonal [EPR1836(2)] to PTP1B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human PTP1B aa 350-450. The exact sequence is proprietary.
Database link: P18031 -
Positive control
- TF1; Jurkat; HepG2 and HeLa cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1836(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PTP1B antibody [EPR1836(2)] (ab133244)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PTP1B knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133244 observed at 54 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133244 was shown to recognize PTP1B when PTP1B knockout samples were used, along with additional cross-reactive bands. Wild-type and PTP1B knockout samples were subjected to SDS-PAGE. ab133244 and ab8245 (loading control to GAPDH) were diluted 1/1,000 and 1/2,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-PTP1B antibody [EPR1836(2)] (ab133244)](https://www.abcam.com/ps/products/133/ab133244/Images/ab133244-146652-anti-ptp1b-antibody-epr18362-western-blot.jpg)
Western blot - Anti-PTP1B antibody [EPR1836(2)] (ab133244)All lanes : Anti-PTP1B antibody [EPR1836(2)] (ab133244) at 1/1000 dilution
Lane 1 : TF1 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa
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![Anti-PTP1B antibody [EPR1836(2)] (ab133244)](https://www.abcam.com/ps/products/133/ab133244/Images/ab133244-4-benefits-of-recombinant-antibodies.png)
Anti-PTP1B antibody [EPR1836(2)] (ab133244)
