Anti-PTP1B antibody [4F8F11] (ab201974)
![Anti-PTP1B antibody [4F8F11] (ab201974)](https://www.abcam.com/ps/products/201/ab201974/Images/ab201974-255528-anti-ptp1b-antibody-4f8f11-western-blot.jpg "Anti-PTP1B antibody [4F8F11] (ab201974)")
Key features and details
- Mouse monoclonal [4F8F11] to PTP1B
- Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PTP1B antibody [4F8F11]
See all PTP1B primary antibodies -
Description
Mouse monoclonal [4F8F11] to PTP1B -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human PTP1B aa 1-250. (Purified from E.coli).
Database link: P18031 -
Positive control
- Human PTP1B (aa40-246) recombinant protein; PTP1B (aa40-246)-hIgGFc transfected HEK293 cell lysate; Jurkat and MCF7 cell lysates; HeLa and Jurkat cells; Human bladder cancer tissue.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR256355-19 are from Tissue Culture Supernatant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4F8F11 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-PTP1B antibody [4F8F11] (ab201974)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PTP1B knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab201974 observed at 54 kDa. Red - loading control, ab181602, observed at 37 kDa.ab201974 was shown to specifically react with PTP1B when PTP1B knockout samples were used. Wild-type and PTP1B knockout samples were subjected to SDS-PAGE. ab201974 and ab181602 (loading control to GAPDH) were diluted 1/500 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-PTP1B antibody [4F8F11] (ab201974)](https://www.abcam.com/ps/products/201/ab201974/Images/ab201974-243314-anti-ptp1b-antibody-4f8f11-western-blot.jpg)
Western blot - Anti-PTP1B antibody [4F8F11] (ab201974)Anti-PTP1B antibody [4F8F11] (ab201974) at 1/500 dilution + Human PTP1B (aa40-246) recombinant protein
Predicted band size: 50 kDaExpected MWt is 50 kDa.
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![Western blot - Anti-PTP1B antibody [4F8F11] (ab201974)](https://www.abcam.com/ps/products/201/ab201974/Images/ab201974-243313-anti-ptp1b-antibody-4f8f11-western-blot.jpg)
Western blot - Anti-PTP1B antibody [4F8F11] (ab201974)All lanes : Anti-PTP1B antibody [4F8F11] (ab201974) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : PTP1B (aa40-246)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 50 kDa
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![Western blot - Anti-PTP1B antibody [4F8F11] (ab201974)](https://www.abcam.com/ps/products/201/ab201974/Images/ab201974-243310-anti-ptp1b-antibody-4f8f11-western-blot.jpg)
Western blot - Anti-PTP1B antibody [4F8F11] (ab201974)All lanes : Anti-PTP1B antibody [4F8F11] (ab201974) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : MCF7 cell lysate
Predicted band size: 50 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [4F8F11] (ab201974)](https://www.abcam.com/ps/products/201/ab201974/Images/ab201974-243308-anti-ptp1b-antibody-4f8f11-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [4F8F11] (ab201974)Immunofluorescent analysis of HeLa cells labeling PTP1B with ab201974 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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![Flow Cytometry - Anti-PTP1B antibody [4F8F11] (ab201974)](https://www.abcam.com/ps/products/201/ab201974/Images/ab201974-243306-anti-ptp1b-antibody-4f8f11-flow-cytometry.jpg)
Flow Cytometry - Anti-PTP1B antibody [4F8F11] (ab201974)Flow cytometric analysis of Jurkat cells labeling PTP1B with ab201974 at 1/200 dilution (green) compared to a negative control (red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [4F8F11] (ab201974)](https://www.abcam.com/ps/products/201/ab201974/Images/ab201974-243305-anti-ptp1b-antibody-4f8f11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [4F8F11] (ab201974)Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling PTP1B with ab201974 at 1/200 dilution folowed by DAB staining.
