Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16314] to STYX - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC, WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-STYX antibody [EPR16314] - BSA and Azide free
    See all STYX primary antibodies

  • Description

    Rabbit monoclonal [EPR16314] to STYX - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251420 is the carrier-free version of ab205200.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    Western blot - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    Anti-STYX antibody [EPR16314] (ab205200) at 1/5000 dilution + Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa


    Exposure time: 1 minute


    This data was developed using ab205200, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    Western blot - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    All lanes : Anti-STYX antibody [EPR16314] (ab205200) at 1/1000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa


    Exposure time: 1 minute


    This data was developed using ab205200, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    Western blot - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    Anti-STYX antibody [EPR16314] (ab205200) at 1/1000 dilution + Human lymph node lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa


    Exposure time: 3 minutes


    This data was developed using ab205200, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    Western blot - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    All lanes : Anti-STYX antibody [EPR16314] (ab205200) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa


    Exposure time: 1 minute


    This data was developed using ab205200, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    Western blot - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    All lanes : Anti-STYX antibody [EPR16314] (ab205200) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa


    Exposure time: 10 seconds


    This data was developed using ab205200, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    Immunocytochemistry - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    This data was developed using ab205200, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling STYX with ab205200 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and weakly cytoplasmic staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab205200 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
  • Immunocytochemistry - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    Immunocytochemistry - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    This data was developed using ab205200, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling STYX with ab205200 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and weakly cytoplasmic staining on Jurkat cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab205200 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
  • Flow Cytometry - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    Flow Cytometry - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    This data was developed using ab205200, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling STYX with ab205200 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    Immunoprecipitation - Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    This data was developed using ab205200, the same antibody clone in a different buffer formulation.STYX was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate with ab205200 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab205200 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: NIH/3T3 whole cell lysate 10ug (Input). Lane 2: ab205200 IP in NIH/3T3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205200 in NIH/3T3 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.
  • Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)
    Anti-STYX antibody [EPR16314] - BSA and Azide free (ab251420)

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