Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIAM2 antibody [EPR16838] - BSA and Azide free (ab232646)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16838] to TIAM2 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TIAM2 antibody [EPR16838] - BSA and Azide free
    See all TIAM2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16838] to TIAM2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human gliocytoma tissue.

  • General notes

    Ab232646 is the carrier-free version of ab199426. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232646 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-TIAM2 antibody [EPR16838] - BSA and Azide free (ab232646)
    Immunocytochemistry/ Immunofluorescence - Anti-TIAM2 antibody [EPR16838] - BSA and Azide free (ab232646)

    Immunocytochemistry/Immunofluorescence analysis of Raw264.7 cells labelling TIAM2 with ab199426 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199426).

  • Flow Cytometry - Anti-TIAM2 antibody [EPR16838] - BSA and Azide free (ab232646)
    Flow Cytometry - Anti-TIAM2 antibody [EPR16838] - BSA and Azide free (ab232646)

    Flow cytometric analysis of 2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling U TIAM2 with ab199426 at 1/450 dilution (red). The secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is Rabbit monoclonal IgG (black) and the cell without incubation with primary antibody and secondary antibody is blue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199426).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAM2 antibody [EPR16838] - BSA and Azide free (ab232646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAM2 antibody [EPR16838] - BSA and Azide free (ab232646)

    Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling TIAM2 with ab199426 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human gliocytoma tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used only secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199426).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-TIAM2 antibody [EPR16838] - BSA and Azide free (ab232646)
    Anti-TIAM2 antibody [EPR16838] - BSA and Azide free (ab232646)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-TIAM2 antibody [EPR16838] - BSA and Azide free (ab232646)

© 2021 Astana Biomed Group LLP. All rights reserved.