Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

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Anti-TIAM2 antibody [EPR16838] (ab199426)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16838] to TIAM2
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-TIAM2 antibody [EPR16838]
See all TIAM2 primary antibodies
Description

Rabbit monoclonal [EPR16838] to TIAM2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Mouse
IHC-P	Human
WB	Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: K562, 293, RAW 264.7, PC-12, NIH/3T3 and C6 whole cell lysates and human fetal brain tissue lysate. IHC-P: Human gliocytoma. ICC/IF: RAW 264.7. Flow Cyt: K562 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16838
Isotype

IgG
Research areas

Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426)

All lanes : Anti-TIAM2 antibody [EPR16838] (ab199426) at 1/10000 dilution

Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Lane 2 : 293 (Human embryonic kidney) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 193 kDa
Observed band size: 193 kDa

Exposure time: 1 minute

Blocking/dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAM2 antibody [EPR16838] (ab199426)

Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling TIAM2 with ab199426 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human gliocytoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used only secondary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TIAM2 antibody [EPR16838] (ab199426)

Immunocytochemistry/Immunofluorescence analysis of Raw264.7 cells labelling TIAM2 with ab199426 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.
Flow Cytometry - Anti-TIAM2 antibody [EPR16838] (ab199426)

Flow cytometric analysis of 2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling U TIAM2 with ab199426 at 1/450 dilution (red). The secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is Rabbit monoclonal IgG (black) and the cell without incubation with primary antibody and secondary antibody is blue.
Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426)

Anti-TIAM2 antibody [EPR16838] (ab199426) at 2000 cells + Human fetal brain lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 193 kDa
Observed band size: 193 kDa

Exposure time: 1 minute

Blocking/dilution buffer: 5% NFDM/TBST.
Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426)

All lanes : Anti-TIAM2 antibody [EPR16838] (ab199426) at 1/1000 dilution

Lane 1 : Rat brain lysate at 10 µg
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 mg/ml
Lane 4 : NIH/3T3 (mouse embryo fibroblast cells) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 193 kDa
Observed band size: 193 kDa

Exposure time: 1 minute

Blocking/dilution buffer: 5% NFDM/TBST.
Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426)

Anti-TIAM2 antibody [EPR16838] (ab199426) at 1/1000 dilution + C6 (rat glial tumor cells) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 193 kDa
Observed band size: 193 kDa

Exposure time: 1 minute

Blocking/dilution buffer: 5% NFDM/TBST.
Anti-TIAM2 antibody [EPR16838] (ab199426)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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