Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIAM2 antibody [EPR16838] (ab199426)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16838] to TIAM2
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TIAM2 antibody [EPR16838]
    See all TIAM2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16838] to TIAM2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, 293, RAW 264.7, PC-12, NIH/3T3 and C6 whole cell lysates and human fetal brain tissue lysate. IHC-P: Human gliocytoma. ICC/IF: RAW 264.7. Flow Cyt: K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16838

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426)
    Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426)
    All lanes : Anti-TIAM2 antibody [EPR16838] (ab199426) at 1/10000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
    Lane 2 : 293 (Human embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 193 kDa
    Observed band size: 193 kDa


    Exposure time: 1 minute


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAM2 antibody [EPR16838] (ab199426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAM2 antibody [EPR16838] (ab199426)

    Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling TIAM2 with ab199426 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human gliocytoma tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used only secondary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TIAM2 antibody [EPR16838] (ab199426)
    Immunocytochemistry/ Immunofluorescence - Anti-TIAM2 antibody [EPR16838] (ab199426)

    Immunocytochemistry/Immunofluorescence analysis of Raw264.7 cells labelling TIAM2 with ab199426 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Flow Cytometry - Anti-TIAM2 antibody [EPR16838] (ab199426)
    Flow Cytometry - Anti-TIAM2 antibody [EPR16838] (ab199426)

    Flow cytometric analysis of 2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling U TIAM2 with ab199426 at 1/450 dilution (red). The secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is Rabbit monoclonal IgG (black) and the cell without incubation with primary antibody and secondary antibody is blue.

  • Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426)
    Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426)
    Anti-TIAM2 antibody [EPR16838] (ab199426) at 2000 cells + Human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 193 kDa
    Observed band size: 193 kDa


    Exposure time: 1 minute


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426)
    Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426)
    All lanes : Anti-TIAM2 antibody [EPR16838] (ab199426) at 1/1000 dilution

    Lane 1 : Rat brain lysate at 10 µg
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 mg/ml
    Lane 4 : NIH/3T3 (mouse embryo fibroblast cells) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 193 kDa
    Observed band size: 193 kDa


    Exposure time: 1 minute


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426)
    Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426)
    Anti-TIAM2 antibody [EPR16838] (ab199426) at 1/1000 dilution + C6 (rat glial tumor cells) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 193 kDa
    Observed band size: 193 kDa


    Exposure time: 1 minute


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Anti-TIAM2 antibody [EPR16838] (ab199426)
    Anti-TIAM2 antibody [EPR16838] (ab199426)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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