Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸ 10 µl 113 243 ₸

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Anti-PHF1 antibody [EPR14222] (ab184951)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14222] to PHF1
Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
Reacts with: Rat, Human

Product name

Anti-PHF1 antibody [EPR14222]
See all PHF1 primary antibodies
Description

Rabbit monoclonal [EPR14222] to PHF1
Host species

Rabbit
Specificity

This antibody is specific to PHD finger protein 1

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- 293, Jurkat, MCF7 and Human fetal brain lysates; Human pancreas and Rat skeletal muscle tissues; MCF7 cells; 293 cells. Mouse and rat brain tissue lysate. NIH/3T3, RAW264.7, C6 and PC-12 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14222
Isotype

IgG
Research areas

Western blot - Anti-PHF1 antibody [EPR14222] (ab184951)

All lanes : Anti-PHF1 antibody [EPR14222] (ab184951) at 1/1000 dilution

Lane 1 : Mouse brain tissue
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 62 kDa
Immunoprecipitation - Anti-PHF1 antibody [EPR14222] (ab184951)

PHF1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab184951 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab184951 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse brain tissue lysate 10 ug

Lane 2: ab184951 IP in Mouse brain tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184951 in mouse brain tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes
Western blot - Anti-PHF1 antibody [EPR14222] (ab184951)

All lanes : Anti-PHF1 antibody [EPR14222] (ab184951) at 1/20000 dilution

Lane 1 : 293 lysate
Lane 2 : Jurkat lysate
Lane 3 : MCF7 lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa
Western blot - Anti-PHF1 antibody [EPR14222] (ab184951)

Anti-PHF1 antibody [EPR14222] (ab184951) at 1/5000 dilution + Human fetal brain lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PHF1 antibody [EPR14222] (ab184951)

Immunofluorescent analysis of MCF7 cells (4% paraformaldehyde-fixed) labeling PHF1 with ab184951 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Flow Cytometry - Anti-PHF1 antibody [EPR14222] (ab184951)

Flow Cytometric analysis of 293 cells (2% paraformaldehyde-fixed) labeling PHF1 with ab184951 at 1/160 dilution (red) mpared to a negative control antibody (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] (ab184951)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] (ab184951)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-PHF1 antibody [EPR14222] (ab184951)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PHF1 antibody [EPR14222] (ab184951)

Key features and details

Overview

Product name

Description

Host species

Specificity

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Images

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