Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PHF1 antibody [EPR14222] (ab184951)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14222] to PHF1
  • Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-PHF1 antibody [EPR14222]
    See all PHF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14222] to PHF1

  • Host species

    Rabbit

  • Specificity

    This antibody is specific to PHD finger protein 1

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293, Jurkat, MCF7 and Human fetal brain lysates; Human pancreas and Rat skeletal muscle tissues; MCF7 cells; 293 cells. Mouse and rat brain tissue lysate. NIH/3T3, RAW264.7, C6 and PC-12 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PHF1 antibody [EPR14222] (ab184951)
    Western blot - Anti-PHF1 antibody [EPR14222] (ab184951)
    All lanes : Anti-PHF1 antibody [EPR14222] (ab184951) at 1/1000 dilution

    Lane 1 : Mouse brain tissue
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate
    Lane 6 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 62 kDa

  • Immunoprecipitation - Anti-PHF1 antibody [EPR14222] (ab184951)
    Immunoprecipitation - Anti-PHF1 antibody [EPR14222] (ab184951)

    PHF1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab184951 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab184951 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 ug

    Lane 2: ab184951 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184951 in mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

     

  • Western blot - Anti-PHF1 antibody [EPR14222] (ab184951)
    Western blot - Anti-PHF1 antibody [EPR14222] (ab184951)
    All lanes : Anti-PHF1 antibody [EPR14222] (ab184951) at 1/20000 dilution

    Lane 1 : 293 lysate
    Lane 2 : Jurkat lysate
    Lane 3 : MCF7 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa

  • Western blot - Anti-PHF1 antibody [EPR14222] (ab184951)
    Western blot - Anti-PHF1 antibody [EPR14222] (ab184951)
    Anti-PHF1 antibody [EPR14222] (ab184951) at 1/5000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PHF1 antibody [EPR14222] (ab184951)
    Immunocytochemistry/ Immunofluorescence - Anti-PHF1 antibody [EPR14222] (ab184951)

    Immunofluorescent analysis of MCF7 cells (4% paraformaldehyde-fixed) labeling PHF1 with ab184951 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow Cytometry - Anti-PHF1 antibody [EPR14222] (ab184951)
    Flow Cytometry - Anti-PHF1 antibody [EPR14222] (ab184951)

    Flow Cytometric analysis of 293 cells (2% paraformaldehyde-fixed) labeling PHF1 with ab184951 at 1/160 dilution (red) mpared to a negative control antibody (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] (ab184951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] (ab184951)

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] (ab184951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] (ab184951)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-PHF1 antibody [EPR14222] (ab184951)
    Anti-PHF1 antibody [EPR14222] (ab184951)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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